Mice derived entirely from embryonic stem (ES) cells can be generated through tetraploid complementation. Although XY male ES cell lines are commonly used in this system, occasionally, monosomic XO female mice produced spontaneous Y chromosome loss. Here, we describe an efficient method to obtain by CRISPR-Cas9-mediated deletion of the chromosome, allowing generation clonal The viable and able ...

Trying to use embryonic stem cells about 20 years ago, working with animals,  especially rats began. During these years , many experiments in mouse embryonic stem cells to transform into a variety of cells and transplanting them were led to remarkable success . In the next issue of human stem cells were considered successful until finally in 1998 the first report was published in the proliferat...

background: the aim of this study was optimization of the polyfect gene delivery method of pcdna3.1 expression vector transfected with the mouse pdx-1 gene in three different kinds of mesenchymal stem cells and hepa cells as well as comparison of transfection efficiency leading to expression of the mentioned gene in the cell types used. materials and methods: rat bone marrow-derived mesenchymal...

The derivation of human embryonic stem cells 10 years ago ignited an explosion of public interest in stem cells, yet this achievement depended on prior decades of research on mouse embryonic carcinoma cells and embryonic stem cells. In turn, the recent derivation of mouse and human induced pluripotent stem cells depended on the prior studies on mouse and human embryonic stem cells. Both human e...

stem cells are undifferentiated biological cells that can differentiate into specialized cells and can divide (through mitosis) to produce more stem cells. they are found in multicellular organisms. in mammals, there are two broad types of stem cells: embryonic stem cells, which are isolated from the inner cell mass of blastocysts, and adult stem cells, which are found in various tissues. in ad...

peroxisomes are small size organelles in nearly all eukaryotic cells involved in various metabolic functions including β-oxidation of very long chain fatty acids and metabolite peroxidation and plasmalogens synthesis. the latter is one of the major constituents in neural cell membranes. the biogenesis of peroxisomes is a complex process which requires multiple proteins encoded by pex genes. dys...

Stem cells are undifferentiated biological cells that can differentiate into specialized cells and can divide (through mitosis) to produce more stem cells. They are found in multicellular organisms. In mammals, there are two broad types of stem cells: embryonic stem cells, which are isolated from the inner cell mass of blastocysts, and adult stem cells, which are found in various tissues. In ad...

Background Degenerative retinal diseases, including age related macular degeneration, glaucoma, and hereditary retinal dystrophies are major causes of blindness. The principal defect in these diseases is cell loss which is amenable to both cell based neuroprotective and neuroregenerative therapies. To briefly review the lines of research and potential candidates for cell based therapies among ...

The niche in which stem cells reside and differentiate is a complex physicochemical microenvironment that regulates cell function. The role played by three-dimensional physical contours was studied on cell progeny derived from mouse embryonic stem cells using microtopographies created on PDMS (poly-dimethyl-siloxane) membranes. While markers of differentiation were not affected, the proliferati...