Stevia rebaudiana Bertoni is a Paraguayan perennial antidiabetic herb belongs to Asteraceae family. The Leaves of stevia are about 300 times sweeter than sugar with no calories, that are the source of diterpene glycosides such as stevioside and rebaudioside A. Tissue culture is a rapid tool for propagation of stevia. In this research we studied the effect of different concentrations of mannitol...

BACKGROUND AND AIMS Cymbidium mastersii is an epiphytic orchid distributed mainly in Northeast India. Owing to its high commercial value in the floricultural industry, natural populations are under threat from over-exploitation. Mass propagation provides an alternative means of satisfying the demand. Unfortunately, conventional propagation is slow and difficult, suggesting in vitro methods for ...

Shoot tips from actively growing, greenhouse maintained plants of Kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on Murashige and Skoog (MS) basal medium supplemented with NAA and BA, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. Results showed that the maximum plantlets height (7.012 cm), node number (4.516), root number (8.860) and roo...

ABSTRACT The genus Melocactus (“cabeça-de-frade”) comprises 32 species in Brazil, of which M. glaucescens and paucispinus are threatened with extinction. present work evaluated the effects different concentrations Murashige & Skoog (MS, MS/2 MS/4) culture medium sucrose (15 g L-1 30 L-1) on vitro seed germination plant growth efficiency sterilization sodium hypochlorite (NaOCl) when using s...

Apical meristems, first, second and third-fourth axillary buds of Ludwigia repens were cultured on Murashige and Skoog (MS) medium containing various concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ) and αnaphthaleneacetic acid (NAA) for micropropagation. Micropropagation was best achieved from apical meristem on MS medium containing 0.05 mg dm TDZ and 0.1 mg dm NAA. It was noted t...

Based on the finite-temperature field theory, we evaluate the medium effects in nucleon which can induce an asymmetry between quarks and antiquarks of the strange sea. The short-distance effects determined by the weak interaction can give rise to δm ≡ ∆ms −∆ms where ∆ms(s) is the medium-induced mass of strange quark by a few KeV at most, but the long-distance effects by strong interaction are s...

Effect of N(6)-benzyladenine (BA) on tanshinone formation in callus cultures of Salvia miltiorrhiza was examined in an attempt to increase the productivity of the medicinal compound, cryptotanshinone. Primary callus was induced by culturing leaf explants on Murashige and Skoog's (MS) basal medium supplemented with 1.0 mg l(-1) of 2,4-dichlorophenoxyacetic acid (2,4-D) in darkness. The callus pr...

The study compared the effects of 1.0 × MS medium containing various concentrations of α-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA), alone or in combination with gibberellic acid (GA3) in micropropagation of three potato (Solanum tuberosum L.) cultivars Pasinler, Granola, and Caspar using binodal stem cuttings. The results testified improved regene...

Two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (Freesia ×hybrida Bailey 'Argenta'). Interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. Using 40% sodium hypochlorite solution for 40 min, cont...