ECVAM sponsored a formal validation study on three in vitro tests for skin irritation, of which two employ reconstituted human epidermis models (EPISKIN, EpiDerm), and one, the skin integrity function test (SIFT), employs ex vivo mouse skin. The goal of the study was to assess whether the in vitro tests would correctly predict in vivo classifications according to the EU classification scheme, "...

OBJECTIVES To establish a reproducible in vitro model for evaluating endothelial cell function in clinical disease states. DESIGN A prospective study of human umbilical vein endothelial cells (HUVEC) isolated and cultured. SETTING Department of Haematology, University of Yaounde I, Cameroon and Department of Medicine University of Newcastle-upon-Tyne, England. RESULTS The optimum initial ...

Background: Susceptibility test is a useful tool for selecting and modifying appropriate treatment for tuberculosis. Objective: To evaluate a direct colorimetric assay based on 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) for a rapid detection of rifampicin resistance. Methods: Sputum was inoculated directly into 7H9 broth supplemented with PANTA (antibiotic mixture) and o...

UNLABELLED Aims/Introduction:  Gastric inhibitory polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) are the major incretins; their secretion after various nutrient loads are well-evaluated in Caucasians. However, little is known of the relationship between incretin secretion and differing nutritional loading in Japanese subjects. In the present study, we evaluated GIP and GLP-1 secretion in...

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Background and Objective: Free radicals cause many diseases in human and their neutralizing by antioxidants reduces risk of cancers. Salvia Officinalis from Lamiaceae family is one of the valuable plants in traditional medicine. The present study aimed to evaluate the antioxidative and cytotoxic properties of ethanolic extract of the aerial parts of this plant on human prostate and cervical can...

The aim of this investigation was to design and develop nanoemulsions as novel delivery systems for rapamycin. Phase behavior of quaternary systems composed of Traicetin (as oil), various surfactants and co-surfactants and water at different surfactant/co-surfactant weight ratios were investigated by the construction of phase diagrams. Formulations were taken from the o/w NE region of the phase...