The effects of various inhibitors on crude, commercial and partially purified commercial mushroom tyrosinase were examined by comparing IC(50) values. Kojic acid, salicylhydroxamic acid, tropolone, methimazole, and ammonium tetrathiomolybdate had relatively similar IC(50) values for the crude, commercial and partially purified enzyme. 4-Hexylresorcinol seemed to have a somewhat higher IC(50) va...

objective: tyrosinase as the key enzyme in melanin biosynthesis, involves in determining the color of mammalian skin and hair. a variety of dermatological disorders, come up from an unnecessary level of epidermal pigmentation. plants are low-cost and rich sources of active compounds.  methods: more than seventy native plants collected from the central region of kurdistan province collected. inh...

The development of melanogenic inhibitors is important for the prevention of hyperpigmentation, and, recently, consideration has been given to natural materials or traditionally used ingredients such as Chinese medicine. The aim of this study is the evaluation of a new anti-melanogenic candidate, kadsuralignan F, from the natural plant Kadsura coccinea, as well as the determination of mechanism...

The mode of action of spent coffee grounds supercritical fluid CO2 extract (SFE) in melanogenesis has never been reported. In the study, the spent coffee grounds were extracted by the supercritical fluid CO2 extraction method; the chemical constituents of the SFE were investigated by gas chromatography-mass spectrometry (GC-MS). The effects of the SFE and its major fatty acid components on mela...

Because it promotes the lightening of pigment spots, tyrosinase inhibition is one mechanisms depigmenting cosmetic products. Considering adverse effects produced by synthetic actives, search for new therapeutic options desirable, and plant extracts are possible candidates hyperpigmentation treatment. Glycolic Cecropia pachystachya Trécul are, therefore, focus this study. Its chemical characteri...

BACKGROUND Mushroom tyrosinase, a copper containing enzyme, modifies growth and survival of tumor cells. Mushroom tyrosinase may foster apoptosis, an effect in part due to interference with mitochondrial function. Erythrocytes lack mitochondria but are able to undergo apoptosis-like suicidal cell death or eryptosis, which is characterized by cell shrinkage and cell membrane scrambling leading t...

Tyrosinase inhibition studies are needed due to the medicinal applications such as hyperpigmentation. For probing effective inhibitors of tyrosinase, a combination of computational prediction and enzymatic assay via kinetics was important. We predicted the 3D structure of tyrosinase, used a docking algorithm to simulate binding between tyrosinase and phthalic acid (PA), and studied the reversib...

Bioassay-guided investigation of the branches of Cleyera japonica led to the isolation of four phenolic constituents: 3,3’-di-O-methylellagic acid (1), 3,3’-di-O-methylellagic acid 4’-O-β-D-xylofuranoside (2), 3,5,7-trihydroxychromone 3-O-α-L-arabinofuranoside (3) and aviculin (4). Their structures were elucidated on the basis of spectral studies, as well as by comparison with literature data. ...

Purpose: To investigate the tyrosinase inhibitory activity and thermostability of weak acid-treated Sophora japonica L. flavonoid complex (SJ-FC) in different solutions. Methods: The flavonoid complex of S. japonica was isolated and treated with weak acid to generate SJ-FC. The anti-tyrosinase activities of SJ-FC and well-known tyrosinase inhibitors were compared by mushroom tyrosinase activity...