Experiments were conducted in chickens to understand the effects of oral immunomodulation. Heat inactivated M phlei, a commensal Mycobacterium and a non-specific immunomodulator, was administered orally prior to live Newcastle disease F (ND F) strain vaccination. In experimental birds it lead to an enhanced cell mediated Immune response (CMI) against the vaccine. There was a reduction in the Ha...

Comparative growth measurements of the mycobacterial species Mycobacterium phlei and Mycobacterium tuberculosis were carried out with liquid cultures supplemented with oxalatoberyllate ions in the concentration range 0-120 microM Be. For both species, the effect of beryllium on specific growth parameters can be represented by a Be concentration-dependent, exponential expression. Evidence for be...

Aim: DNA topoisomerases control several cellular activities by catalyzing the relaxation of superhelices, which are produced during the replication of DNA. Investigation of the inhibitory action of the candidate substances on topoisomerase activity is widely used in drug development. The purpose of this study was to purify topoisomerase I from Mycobacterium phlei, which is closely related to My...

Edson (1947, 1951), however, pointed out that further purification would be needed before this hypothesis could be tested satisfactorily. A later report showed that the purified enzyme was not a typical flavoprotein (Edson & Cousins, 1953). Since lactate-oxidizing enzymes resembling the enzyme of Myco. phlei have been extracted from Mycobacterium sregmati8, Mycobacterium tuberculo8i8 var. homin...

Mycobacterium &megmati at a concentration of 1: 20,000 and Myco. phlei at 1:10,000 but was completely inactive against an unidentified species of Mycobacterium not listed in Table 1. Fusein-thioglycollic acid. The results obtained. are summarized in Table 2. They indicate that, in general, the conversion of fuscin into its thioglycollic acid derivative caused a remarkably small change in its an...

A facultatively methylotrophic Mycobacterium was isolated from Cleveland Harbor, Ohio, USA. The isolate, designated ID-Y, used a wide range of carbon and energy sources including methane and several other hydrocarbons. It displayed a growth cycle from rod-shaped exponential-phase cells, with many cell pairs exhibiting V-formation, to cocco-bacillary stationary-phase cells. A fixation technique ...

The dihydrofolate reductase from Mycobacterium phlei was purified and characterized; it has an Mr of 15 000 and a pI of 4.8. It is competitively inhibited by both methotrexate and trimethoprim, although the affinity is less than for other bacterial dihydrofolate reductases.

objective(s): th2 response is related to the aetiology of asthma, but the underlying mechanism is unclear. to address this point, the effect of nebulized inhalation of inactivated mycobacterium phlei on modulation of asthmatic  airway  inflammation was investigated. materials and methods: 24 male balb/c mice were randomly divided into three groups: control group (group a), asthma model group (g...