In summary, in order to control tuberculosis, we require a rapid diagnosis of TB patients, who act as the source of disease; and if remain untreated, these patients can spread the disease throughout the population. Therefore, we need to introduce the new approach for quick and accurate identification of Mycobacterium tuberculosis, and CYP141 gene is a new approach for rapid and accura...

Tuberculosis and Crohn disease are granulomatous disorders affecting the intestinal tract with similar clinical manifestations and pathologic features. We evaluated the use of in situ polymerase chain reaction (PCR) using Mycobacterium tuberculosis complex-specific primers for IS 6110 to differentiate these 2 disorders in archival mucosal biopsy specimens. In situ PCR was positive in 6 of 20 tu...

Herein we report a 6-year-old Afghans patient referred to Department of Dermatology, St. AL-Zahra Hospital in Isfahan with ulcerative and nodular lesions disseminated over the face, arms, buttocks, pelvic girdle and legs in 2002. Direct smears of the lesions were positive for Leishman bodies. Leishmanin skin test was negative. He also had dactylitis in some digits and toes. PCR from bone biopsy...

Background: Tuberculosis (TB) is a curable infectious disease yet the leading cause of death worldwide and in Bangladesh it is responsible for 7% of total deaths every year. Objectives: This cross sectional study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of polymerase chain reaction (PCR) for the early diagnosis of pulmonary tuberc...

BACKGROUND Little information is available on the molecular epidemiology in Mexico of Mycobacterium species infecting extrapulmonary sites in humans. This study used molecular methods to determine the Mycobacterium species present in tissues and body fluids in specimens obtained from patients in Mexico with extrapulmonary disease. METHODS Bacterial or tissue specimens from patients with clini...

The worldwide recrudescence of tuberculosis and the widespread appearance of antibiotic resistance have strengthened the need for rapid and specific diagnostic tools. The prevailing microbiological identification of Mycobacterium tuberculosis, the causative agent of tuberculosis, which implies the use of in vitro cultures and acid-fast staining microscopy, is time-consuming. Detection of M. tub...