1. Carbon dioxide-bicarbonate mixtures markedly inhibited oxidation and phosphorylation rates of mitochondria prepared from cauliflower. Inhibition occurred with succinate, malate, citrate, isocitrate and NADH as substrates. 2. Indophenol-reductase systems with malate, succinate, isocitrate and NADH as substrates were inhibited by 5% and 15% carbon dioxide. Cytochrome c oxidase was not inhibite...

The growth of Lactobacillus delbrueckii subsp. bulgaricus (L. delbrueckii subsp. bulgaricus) on lactose was altered upon aerating the cultures by agitation. Aeration caused the bacteria to enter early into stationary phase, thus reducing markedly the biomass production but without modifying the maximum growth rate. The early entry into stationary phase of aerated cultures was probably related t...

Amphibacillus xylanus Ep01, a facultative anaerobe we recently isolated, shows rapid aerobic growth even though it lacks a respiratory pathway. Thus, the oxidative consumption of NADH, produced during glycolysis and pyruvate oxidation, should be especially important for maintenance of intracellular redox balance in this bacterium. We purified a flavoprotein functional as NADH oxidase from aerob...

The sensitivity of nicotinamide adenine dinucleotide (NADH) oxidase and succinoxidase to metal chelators, the generation of an electron paramagnetic resonance (EPR) signal upon addition of these substrates, and the rate of formation of the EPR signal relative to the rate of the cytochrome reduction suggest the participation of nonheme iron proteins in the respiratory process of Escherichia coli...

Dowler, William M. (University of Illinois, Urbana), Paul D. Shaw, and David Gottlieb. Terminal oxidation in cell-free extracts of fungi. J. Bacteriol. 86:9-17. 1963.-The terminal respiration in cell-free extracts of ten representative fungi is mediated by an electron transport system similar to that observed in animal tissue. Reduced nicotinamide adenine dinucleotide (NADH) and succinic cytoch...

In peroxisomes isolated from pea leaves (Pisum sativum L.) the production of superoxide free radicals (O(2) (-)) by xanthine and NADH was investigated. In peroxisomal membranes, 100 micromolar NADH induced the production of O(2) (-) radicals. In the soluble fractions of peroxisomes, no generation of O(2) (-) radicals was observed by incubation with either NADH or xanthine, although xanthine oxi...

The sensitivity of nicotinamide adenine dinucleotide (NADH) oxidase and succinoxidase to metal chelators, the generation of an electron paramagnetic resonance (EPR) signal upon addition of these substrates, and the rate of formation of the EPR signal relative to the rate of the cytochrome reduction suggest the participation of nonheme iron proteins in the respiratory process of Escherichia coli...

AIMS To evaluate the mitochondrial respiratory enzyme activities in blood cells of Leber's hereditary optic neuropathy (LHON) with 11778 point mutation of mitochondrial DNA. METHODS Assays for the activities of NADH-cytochrome c reductase (complex I+complex III), succinate-cytochrome c reductase (complex II+complex III), and cytochrome c oxidase (complex IV) on blood cell mitochondria of seve...

Reactive oxygen species (ROS) including superoxide anions (O2(-)) play a key role in atherogenesis, and endothelial cells have the ability to generate ROS. To investigate the enzymatic sources of ROS and the effects of lysophosphatidylcholine (LPC), an atherogenic lipid, we measured ROS production in cultured bovine aortic endothelial cells (BAECs) by the lucigenin-enhanced chemiluminescence (C...