Myoblasts, the contractile cells of skeletal muscle, have been invaluable for fundamental studies of muscle development and clinical applications for muscle loss. A major limitation to the myoblast-based therapeutic approach is contamination with non-contractile fibroblasts, which overgrow during cell expansion. To overcome these limitations, this study was carried out to establish a 3D culture...

objective: in this study we introduced an rgd-containing peptide of collagen iv origin that possesses potent cell adhesion and proliferation properties. this peptide was immobilized on a nanofibrous polycaprolactone/gelatin scaffold after which we analyzed human bone marrow-derived mesenchymal stem cells (hbmscs) adhesion and proliferation on this peptide-modified scaffold. methods: nanofibrous...

one of the main issues of nanotechnology is producing materials with new properties. nanotechnology, as a powerful tool, has the ability to create evolution in the agricultural system and food–medicinal industries across the world. producing a high-performance material from reclaimed cellulose material will increase motivation to recycle these materials at all phases of paper production and rem...

the main purpose of this study is to introduce a new method for nanofibers by bulk layer production. in the present method the effect of some parameters including take-up velocity, applied voltage, polymer solution concentration on density of produced layers is investigated. the compression characteristic of nanofiber layers produced under optimum electrospinning is also investigated. it was sh...

We report the design, fabrication, and characterization of novel, low-cost, and modular miniaturized nanofiber electrospinning sources for the scalable production of non-woven aligned nanofiber mats with low diameter variation. The devices are monolithic arrays of electrospinning emitters made via stereolithography; the emitters are arranged so each element has an independent line of sight to a...

objective: the development of combining mesenchymal stem cells (mscs) with surface modified three-dimensional (3d) biomaterial scaffold provides a desirable alternative for replacement of damaged and diseased tissue. nanofibrous scaffolds serve as suitable environment for cell attachment and proliferation due to their similarity to the physical dimension of the natural extracellular matrix. in ...

objective(s): in this work, electrospun nanofibers were explored as drug delivery vehicles using tetracycline as a model drug.  nanocomposite fibers including chitosan (cs)/poly (ethylene oxide) (peo) and antibiotic were successfully prepared using electrospinning. cs blended with peo considering a weight ratio of (90/10), and then, nanofibrous samples were successfully electrospun from their a...

In this study, a blend mixture of chitosan-ethylenediaminetetraacetic acid (CS 2 wt%-EDTA) at a weight ratio of 30/70 and polyvinyl alcohol (PVA) solution (10 wt%) was electrospun to produce fibrous mats with lysozyme (10, 20 and 30 wt%) used for wound healing. The morphology and diameter of the electrospun fiber mats with and without lysozyme were analyzed by scanning electron microscopy (SEM)...

Cardiac failure is a quite severe condition that can result in life-threatening consequences. Cardiac tissue engineering is thought to be one of the most promising technologies to reconstruct damaged cardiac muscles and facilitate myocardial tissue regeneration. We report a new nanofiber bundle substrate for three-dimensional (3D) cardiac cell culture as a platform to investigate cell morpholog...