From the time the Leishman stain has been used for staining blood cells and parasites, it has been dissolved in acetone-free good quality methyl alcohol. Dissolving the stain in inferiorquality methyl alcohol or ethyl alcohol always gives poor results and hsematologists and parasitologists requiring precision work would always use acetone-free methyl alcohol of the best quality. After the secon...

A fluorescent nucleic acid stain that does not penetrate living cells was used to assess the integrity of the plasma membranes of bacteria. SYTOX Green nucleic acid stain is an unsymmetrical cyanine dye with three positive charges that is completely excluded from live eukaryotic and prokaryotic cells. Binding of SYTOX Green stain to nucleic acids resulted in a > 500-fold enhancement in fluoresc...

Aim: A reliable diagnosis of Helicobacter pylori (H. pylori) is important in clinical practice and research. The aim this study was to compare the sensitivity specificity Giemsa stain with hematoxylin eosin (H&E), Toluidine blue, Gimenez Warthin-Starry detection gastric biopsy also immunohistochemistry (IHC) H. organism biopsy. Method: retrospective cross-sectional included 200 formalin-fix...

“Is it possible to predict expression levels of different genes at a given spatial location in the routine histology image tumor section by modeling its stain absorption characteristics?” In this work, we propose “stain-aware” machine learning approach for prediction transcriptomic gene profiles using digital pathology Hematoxylin & Eosin (H&E) section. Unlike recent deep methods which are used...

To optimize diagnosis of histoplasmosis in tissue sections, 30 spleen specimens from mice, experimentally infected with Histoplasma capsulatum, were examined by H&E, Grocott stain, anti-bacille Calmette-Guerin antibody immunostain, Fungiqual A fluorochrome stain (Drs Reinehr and Rembold, Kandern, Germany), and a nested polymerase chain reaction (PCR) assay. Results were compared with the tissue...

A major drawback to clinicians prescribing and patients using chlorhexidine rinse is the development of extrinsic staining. In order to assess the effectiveness of a whitening dentifrice in controlling chlorhexidine stain, fifty-seven subjects rinsed with chlorhexidine twice daily while brushing twice daily with either fluoridated whitening or a fluoridated regular dentifrice. Stain was assesse...