BACKGROUND The International Staging System for multiple myeloma has increased the importance of accurate measurement of serum albumin. Two common albumin assays, bromcresol green (BCG) and agarose gel protein electrophoresis (PEL), frequently yield discordant results, creating confusion regarding which assay is superior for use in myeloma. METHODS We measured albumin by BCG on a Roche Modula...

Objective: To evaluate the diagnostic usefulness of a gelatin based Waaler Rose assay (Serodia-RAH) for the detection of rheumatoid factor (RF) in rheumatoid arthritis (RA). Methods: RF was measured by Serodia-RA and rate nephelometry in 90 patients with RA according to the revised ACR criteria and 102 patients with rheumatological diseases other than RA. Sensitivity, specificity, accuracy, lik...

OBJECTIVE The objective of this study was to develop a reproducible method of establishing the concentration of yeast cells per milliliter of solution. METHODS Three methods of determining the number of yeast cells in solution were compared: Neubauer's counting chamber, spectrophotometry, and nephelometry. RESULTS All three methods were comparable and reproducible. The following formulas we...

IgM rheumatoid factor was assayed by three routine methods: latex fixation; haemagglutination; and end point laser nephelometry in 69 patients with definite or classical rheumatoid arthritis and 58 patients with other non-rheumatoid arthropathies, selected prospectively according to the American Rheumatism Association clinical criteria. The operators of the assays were unaware of the clinical d...

OBJECTIVE To evaluate the diagnostic usefulness of a gelatin based Waaler Rose assay (Serodia-RA) for the detection of rheumatoid factor (RF) in rheumatoid arthritis (RA). METHODS RF was measured by Serodia-RA and rate nephelometry in 90 patients with RA according to the revised ACR criteria and 102 patients with rheumatological diseases other than RA. Sensitivity, specificity, accuracy, like...

BACKGROUND Chronic obstructive pulmonary disease (COPD) is an inflammatory pulmonary disorder with systemic inflammatory manifestations that are mediated by circulating acute-phase reactants. This study compared an enzyme-linked immunosorbent assay (ELISA) to a nephelometric technique for the measurement of serum C-reactive protein (CRP) and serum amyloid A (SAA) and investigated how the choice...

We describe a simple approach for assaying immune complexes from serum by usinganti-lgG as the indicatorafter a three-step extraction procedure with polyethylene glycol. Analysisof the data indicatesthat assaysof suchextractsfor immune complexes by absorbance nephelometry, kinetic light scatter, and immunoradiometric techniques correlate well. For 116 samples, results by absorbance nephelometry...

Immunoglobulins G, A, and M were measured by radial immunodiffusion and by laser nephelometry in sera from 33 patients and in a preparation of purified colostral IgA. In general the results agreed for the two methods, with some exceptions attributed to the physicochemical characteristics of the immunoglobulins assayed: low-molecular-weight (monomeric) IgM gives falsely high values and IgA prote...