Determination of apoB by rate nephelometry is a simple, rapid and precise method, which is also suitable for a routine laboratory. It can be standardized with lipoprotein-B or LDL fractions, but only if these fractions are diluted with fresh whole serum. The standardization is also valid for VLDL if the samples are diluted with hydroxypolyethoxydodecane (trade name: Thesit) in a concentration o...

This work is based on the well-established immunoassay principle of agglutination of latex particles covered by immunoproteins. In our experiments, positively charged particles act as carriers for the F(ab')2 fragment, obtained from rabbit polyclonal IgG, active against C-reactive protein (CRP). The presence of the antigen CRP in the immunolatex system causes agglutination and the aim of the pr...

We studied the development of light scattering in the reaction between anti-apolipoprotein B and apolipoprotein B in intact very-low-density lipoproteins (I) and low-density lipoproteins (II) as well as in lipoproteins treated with lipases, and found considerable differences in the kinetics of the immunoreaction for the two lipoprotein classes. Pre-incubation with triglyceride lipase and choles...

Ever since the description by Green' of a modification of the colorimetric method reported by Soria2 to determine the kinetics of fibrin polymerisation induced by reptilase was published, no attempts have been made to improve the sensitivity of the method. The controversial association of the abnormalities of fibrin polymerisation detected by traditional methods with the presence and severity o...