Local reference ranges of immunoglobulins are required for studies and clinical interpretation. In this study, serum levels of IgA, IgG, and IgM in 914 Iranian healthy adult blood donor volunteers, aged 18-55 years, were measured by nephelometry. Our data showed that serum reference intervals of IgA, IgG, and IgM in subjects were 72-375, 636-1518, and 39-283 mg/dL, respectively. Data analysis s...

We describe turbidimetric methods for simultaneously determining C-reactive protein and orosomucoid in 33 microL of undiluted serum in the Technicon RA-1000, with multipoint calibration. Precision is good and results correlate favorably with those of nephelometry with the Beckman ICS (r = 0.98 for CRP, 0.99 for orosomucoid). Analyses are easy to perform, require infrequent calibration, and are ...

Hydatid disease is caused by Echinococcus granulosus. In this study, we aimed to investigate the benefit of monitoring cases with hydatid cyst by means of immune components in patients in a long-term follow-up after surgery. Eighty-four preoperative and postoperative serum samples from 14 cases undergoing surgery for hydatid disease were evaluated in terms of immune parameters, such as total an...

MATERIAL AND METHODS: At the Institute of Immunobiology and Human Genetics, part of the Faculty of Medicine in Skopje, in the previous 7 years, total of 361 patients with suspected alpha-1-antitrypsin (AAT) deficiency were referred for analysis of AAT concentration using nephelometry (Dade Behring) and subsequent AAT genotyping of individuals with alpha-1antytripsin deficiency at protein level,...

The methods currently available for quantitative determination of lysozyme are based on the capacity of the enzyme to lyse Micrococcus lysodeikticus. Previous attempts to develop immunochemical assays have been reported, but the sensitivity was inferior to that of the most widely used lysoplate method. We describe the development of a sensitive immunochemical method for lysozyme assay in which ...

The Z deficiency in α1-antitrypsin (A1ATD) is an under-recognized condition. Alpha1-antitrypsin (A1AT) is the main protein in the α1-globulin fraction of serum protein electrophoresis (SPE); however, evaluation of the α1-globulin protein fraction has received very little attention. Serum Z-type A1AT manifests in polymeric forms, but their interference with quantitative immunoassays has not been...

Quantitative measurement of C-reactive protein (CRP) in serum has been proposed as a sensitive and, for some populations, a specific indicator of infection. To determine whether early measurement of CRP in serum could differentiate patients with bacteremia from a control group of patients whose blood cultures yielded contaminants, we measured CRP concentrations quantitatively by rate nephelomet...