نتایج جستجو برای: nested PCR-RFLP

تعداد نتایج: 202759  

Journal: :The Indian journal of medical research 2007
P Sowmya V Dhanya H N Madhavan K L Therese

BACKGROUND & OBJECTIVE Glycoprotein B (gB), involved in cell-to-cell transmission of human cytomegalovirus (HCMV), is a critical factor in tissue tropism and viral pathogenesis. The aim of the present study was to compare the efficiency of PCR-based RFLP and multiplex nested PCR for gB gene of HCMV to determine their genotype in clinical specimens from patients with HCMV. METHODS The PCR base...

Journal: :The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases 2011
Janaina Coser Thaís da Rocha Boeira André Salvador Kazantzi Fonseca Nilo Ikuta Vagner Ricardo Lunge

BACKGROUND It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene. METHODS Analysis of published DNA sequence of mucosal HPV types to select sequences of new...

Journal: :iranian journal of parasitology 0
vahid noaman parviz shayan narges amininia

background: anaplasmosis belongs to the complex of several tick-borne diseases and can cause diseases in the livestock with high economical losses. cattle that recover from acute infection become carriers and the parasite can persist most probably for the lifetime in the blood. the aim of the present study was the determination of the persistently infected cattle in a region of iran with the pr...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه پیام نور - دانشگاه پیام نور استان تهران - دانشکده علوم پایه 1390

تعیین گونه های عامل لیشمانیوز پوستی به روش مولکولی pcr-nested و rflpدرشهرستان جهرم. لیشمانیوز یکی از بیماریهای انگلی می باشد که توسط تک باخته ای از جنس لیشمانیا ایجاد می شود. این بیماری به صورت گسترده در مناطق مختلفی از جهان مشاهده می شود . بیماری دارای طیف وسیعی بوده که در یک طرف آن زخمی ساده است و در طرف دیگر طیف اشکال پوستی ، پوستی مخاطی ، و لیشمانیوز احشایی می باشد که حتی می تواند منجر به ...

Journal: :Ege Üniversitesi Ziraat fakültesi dergisi 2022

Objective: The objective of this study was to investigate the group/subgroup phytoplasma agent in peppers showing symptoms. Material and Methods: In study, plants collected from Iğdır province 2020 were analyzed using direct nested PCR tests, BLASTn, iphyClassifier, Mega 7, pDRAW32 programs used. Results: tests performed, approximately 1.2 kb DNA fragments specific obtained. 16S rRNA nucleotide...

Journal: :Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2005
E C Castelli D S Gil L C S Veiga J L V de Camargo

In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial ...

ژورنال: :پژوهش های آسیب شناسی زیستی 0
laleh yazdani department of virology, faculty of medical sciences, tarbiat modares university, tehran, iran mehrdad ravanshad department of virology, faculty of medical sciences, tarbiat modares university, tehran, iran zahra khanlari department of virology, faculty of medical sciences, tarbiat modares university, tehran, iran ramin yaghoobi shiraz transplant research center, shiraz university of medical sciences, shiraz, iran

هدف: gb ویروس c جزء ویروس‏های منتقل شونده از راه خون، فرآورده‏های خونی و از طریق جنسی است و از آن‏جا که راه انتقال آن با ویروس‏های عامل هپاتیت مشترک است، طی این مطالعه ویروس را در بین افراد hbsag مثبت با روش حساس‏تر semi nested-pcr جداسازی کرده و ژنوتیپ‏های ویروس با روش rflp تعیین شد. مواد و روش‏ها: در تحقیقی که به‏صورت مقطعی بین سال‏های 1389 تا 1390، روی 100 نمونه سرمی افراد hbsag مثبت انجام ش...

Journal: :Applied and environmental microbiology 2005
Yoshitsugu Ochiai Chieko Takada Mitsugu Hosaka

Cryptosporidium parvum and C. hominis have been the cause of large and serious outbreaks of waterborne cryptosporidiosis. A specific and sensitive recovery-detection method is required for control of this pathogen in drinking water. In the present study, nested PCR-restriction fragment length polymorphism (RFLP), which targets the divergent Cpgp40/15 gene, was developed. This nested PCR detecte...

Journal: :modares journal of medical sciences: pathobiology 2012
laleh yazdani mehrdad ravanshad zahra khanlari ramin yaghoobi

objective: gbv-c is a virus transmissible via blood transfusion and sexual routes. because of the shared transmission routes in gbv-c and hepatitis b virus (hbv) we have attempted to detect gbv-c in hbsag-positive patients using the more sensitive semi-nested pcr. we used restriction fragment length polymorphism (rflp) to determine genotype. methods: a total of 100 serum samples were collected...

Journal: :iranian journal of parasitology 0
k manouchehri naeini department of parasitology, mycology and entomology, school of medicines, shahr-e-kord university of medical science, shahr-e-kord, iran m asadi department of parasitology, mycology and entomology, school of medicines, shahr-e-kord university of medical science, shahr-e-kord, iran m hashemzade chaleshtori cellular and molecular research center, shahr-e-kord university of medical sciences, shahr-e-kord, iran

background: the aim of this study was to detect and characterize cryptosporidium spp. in water sam­ples collected from recreational ponds of chaharmahal va bakhtiyari province of iran . meth­ods: thirty water samples were collected  from november 2009 to may 2010. each sample con­tained 10 liters of water. we used the ssu rrna-based pcr-rflp technique. results: out of thirty samples examined, 6...

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