Neutrophils are professional phagocytes that migrate early, in high number, to the infection sites. Our study has analyzed how neutrophils cross-present antigens and influence CD8+ T-cell responses. By using highly purified neutrophils from peritoneal exudates and bone marrow, we have shown that neutrophils cross-present ovalbumin to a CD8+ T-cell hybridoma and to naive CD8+ T cells from OT1 tr...

Essential cells of innate immunity, neutrophils are often considered to be a homogenous population of terminally differentiated cells (Chakravarti et al., 2009). These cells represent the body’s primary line of defence against invading pathogens such as bacteria, and constitute 40-60% of the white blood cell population. Neutrophils are short-lived polymorphonuclear phagocytes. They are known as...

The kinetics of sodium dodecyl sulfate-induced activation of respiratory burst oxidase (NADPH oxidase) in a fully soluble cell-free system from resting (control) or phorbol myristate acetate (PMA)-stimulated human neutrophils were investigated. In a cell-free system containing solubilized membranes and cytosol fractions (cytosol) derived from control neutrophils (control cell-free system), the ...

BACKGROUND The role of phototherapy on neutrophils has not been reviewed previously. This novel and non-invasive therapeutic approach is of particular interest for potential use in the treatment of pathologic processes in dermatology and infectious diseases in which neutrophils are the primary culprit. OBJECTIVES The primary aim of this study was to systematically review the role of photother...

Phagocytosis of apoptotic cells, also called efferocytosis, is an essential feature of immune responses and critical for the resolution of inflammation. Plasma and tissue levels of plasminogen activator inhibitor-1 (PAI-1), an inhibitor of fibrinolysis, are elevated in inflammatory conditions, including sepsis and acute lung injury, in which activated neutrophils accumulate in tissues and contr...

The kinetics of sodium dodecyl sulfate-induced activation of respiratory burst oxidase (NADPH oxidase) in a fully soluble cell-free system from resting (control) or phorbol myristate acetate (PMA)-stimulated human neutrophils were investigated. In a cell-free system containing solubilized membranes and cytosol fractions (cytosol) derived from control neutrophils (control cell-free system), the ...

We conducted the following study to determine if bispecific antibodies enhance the bactericidal activity of bovine neutrophils. Bispecific antibodies were synthesized by chemically crosslinking bovine neutrophil monoclonal antibodies to Staphylococcus aureus 305 capsule polysaccharide monoclonal antibodies. The efficiency of chemically coupling monoclonal antibody monomers was approximately 50%...

Intracellular pathogens are mainly eliminated by cell-mediated immunity offered phagocytic cells like neutrophils, macrophages and monocytes. Two tannin glycosides, 1-O-galloyl-6-O-luteoyl-α-D-glucopyranoside (1) 3-O-methylellagic acid 3'-O-α-rhamnopyranoside (2) were isolated from the ethyl acetate fraction (EF) of methanol leaf extract Phyllanthus muellerianus. The structures compounds elucid...

Aim. To assess external respiration (ER) and its relationship with the activity of enzymes involved in purine metabolism patients acute chronic forms pulmonary tuberculosis (TB). Materials methods. In TB, we assessed adenosine deaminase (ADA)-1, 2 blood serum (eADA), mononuclear cells, neutrophils, concentration ecto-5’-nucleotidase (eNT5E) serum, level CD26 (dipeptidyl peptidase-4, DPPIV) prod...

thirty clinical cases of obstructive urolithiasis, 15 with intact urinary bladder and 15 with ruptured urinary bladder, were used to study the changes in peritoneal fluid cytology. the percentage of neutrophils was highly increased than the normal reference range reported for cattle with slight decrease in lymphocyte percentage. the value for neutrophil percent was almost similar in both intact...