total protein and dna extracted from histologically diagnosed normal nonmalignant and esophageal tumor tissues were used for analysis of polypeptides pattern by two-dimensional gel electrophoresis and dna transforming activity in nih 3t3 cell transfection assay, respectively. in comparison to normal tissues, eight polypeptides underwent down-regulation or disappeared, while seven polypeptides w...

Extremely large datasets have become routine in biology. However, performing a computational analysis of a large dataset can be overwhelming, especially for novices. Here, we present a step-by-step guide to computing workflows with the biologist end-user in mind. Starting from a foundation of sound data management practices, we make specific recommendations on how to approach and perform comput...