apotransferrin (apo tf)  in 0.1 m n-(2hydroxyethyl)piperazine-n2-ethanesulfanic acid at 25 ˚c  and ph 7.4 has been titrated with acidic solution of tb3+. the binding of tb3+ at  the two specific metal-binding sites of transferrin was followed from the changes in the difference uv spectra at 245 nm. the molar absorptivity per binding site for tb3+-tf is 22,500 ± 1000 m-1cm-1. to determine the tb...

Metal dissociation constants for glyoxalase I from human erythrocytes were determined by use of nitrilotriacetic acid as a metal buffer. The constants for Zn2+, Co2+, Mn2+, and Mg2+ were (2.7 +/- 0.3) X 10(-11) M, (3.0 +/- 0.8) X 10(-10) M, (4.9 +/- 0.5) X 10(-9) M, and (1.0 +/- 0.2) X 10(-6) M, respectively, demonstrating that the natural cofactor, Zn2+, has the highest affinity for the apopro...

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Bioassays for the carcinogenicity of nitrilotriacetic acid, trisodium salt, monohydrate (Na3-NTA-H2O) were conducted at Stanford Research Institute (SRI), using Fischer 344 rats and at Litton Bionetics, Inc. (LBI), using both Fischer 344 rats, and B6C3F1 mice. Similar bioassays using rats and mice, were conducted at LBI on the free acid, nitrilotriacetic acid (NTA). Each chemical was mixed in r...

Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergents and perform a detergent-lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron reflection using the predicted model from molecular dynamic simulations.