The high-performance liquid-chromatographic method described here for the quantitative analysis for nitrofurantoin in urine and plasma involves direct analysis of urine samples and analysis of plasma samples after protein precipitation by methanol. The assay, which requires only 0.2 ml of biological fluid and shows a linear relationship in the range 0.02 to 200 mg/liter, can be performed in 9 m...

The title compound, C(12)H(11)N(3)O(2), adopts an E or trans configuration with respect to the C=N bond. There is an intra-molecular O-H⋯N hydrogen bond involving the hydroxyl H atom and an N atom of the hydrazine group. In the crystal structure, mol-ecules are connected via N-H⋯O hydrogen bonds, forming a three-dimensional network.

Wild-type Escherichia coli cells are sensitive to nitrofurazone (NF) and many other nitrofuran derivatives. A variety of evidence indicated that these compounds are converted to toxic "active" metabolites by reductases present in the bacteria. Sensitive E. coli K-12 acquired threefold-greater resistance to NF in one mutational step. These partially resistant mutants could undergo a second mutat...

Cultured epidermal skin has become an adjunctive therapy for treatment of major burn injuries, but its effectiveness is greatly limited because of destruction by microbial contamination. To evaluate candidate antimicrobial agents for use with cultured skin, a combined cytotoxicity-antimicrobial assay system was developed for determination of toxicity to cultured human keratinocytes and fibrobla...

Avian strains of Escherichia coli (213) were subjected to in vitro antibiotic sensitivity tests with twenty-four different chemotherapeutic agents. Chlorhexidine, polymyxin B, colistin, and nitrofurazone exhibited the greatest inhibitory activities against these strains. Various potencies of antibiotics were compared. The activity of sulfachloropyridazine on various types of media failed to sho...

The wound healing activity of ethanol extract of Terminalia bellirica Roxb. fruit was evaluated on excision and incision wound model, in albino rats, in the form of an ointment with two concentrations (2 and 4% w/w ointment) of fruit extract in simple ointment base. Both concentrations of the ethanol extract showed significant response in both the wound types tested when compared with the contr...