Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for characteriza‐ tion of materials. It can detect local structure around selected atomic nuclei and provide information on the dynamics of these nuclei. In case of metal-organic frameworks, NMR spectroscopy can help elucidate the framework structure, locate the molecules adsorbed into the pores, and inspect and characterize the i...

The most studied physiological role of Tau is the stabilization of microtubules. However, how this function is performed, is still unknown. We will present results based on NMR spectroscopy with Tau and a stathmin stabilized soluble tubulin form that give mechanistic insights in this process. Phosphorylation regulates Tau’s function in a complex manner. Combining in vitro phosphorylation and NM...

We report the first direct solution NMR detection of the alkali metal cations (23Na+, 39K+, and 87Rb+) residing inside G-quadruplex channel structures formed by guanosine 5'-monophosphate and a DNA oligomer, d(TG4T). In solution, these channel alkali metal cations are tightly bound to the G-quadruplex structure and have been considered to be "invisible" to NMR spectroscopy for many years. Our f...

Using NMR spectroscopy and NMR imaging, the behaviour of polymers in supercritical CO2 (sc-CO2) using a custom-made cell designed from poly(ether ether ketone) (PEEK) has been observed. The mechanism of swelling of polymers in sc-CO2 and the overall effects of sc-CO2 on the transport properties of both small and large molecules has been studied. We report on the swelling characteristics of radi...

Nuclear Magnetic Resonance spectroscopy and imaging can be classified as inductive techniques working in the near- to far-field regimes. We investigate an alternative capacitive detection with the use of micrometer sized probes positioned at sub wavelength distances of the sample in order to characterize and model evanescent electromagnetic fields originating from NMR phenomenon. We report that...

Studies on the chemical constituents of roots of Urtica triangularis HAND-MASS have led to the isolation of four new compounds. The structures, including the absolute configurations, of these constituents have been elucidated through spectral studies including (1)H-NMR, (13)C-NMR, 2D-NMR experiments (heteronuclear single-quantum coherence, heteronuclear multiple bonding connectivity and nuclear...

Recent applications of high-field NMR spectroscopy in biosynthesis will be discussed. The emphasis of the lecture will be on the use of 1H, 3H, '3C and '5N-NMR to focus on individual steps of biochemical processes in the formation of natural products and related enzyme-catalyzed events. In the first set of experiments, pulse labelling and invivo studies are used to uncover biosynthetic sequence...

High-resolution (1)H NMR spectroscopy of body fluids has proved to be very useful in diagnostics of inherited metabolic diseases, whereas (13)C NMR remains almost unexploited. In this paper the application of (13)C NMR spectroscopy of fivefold concentrated urine samples for diagnosis of selected metabolic diseases is reported. Various marker metabolites were identified in test urine samples fro...

The power of Nuclear Magnetic Resonance spectroscopy (NMR) in structure elucidation derives in large part from its ability to establish bonding connectivity (via Jcoupling interaction) or through space proximity (via dipolar coupling interactions) of nuclei. The amount of time consumed in elucidating a structure depends on the rate at which these interaction can be detected by NMR and analyzed....

Magnetic resonance (MR) spectroscopy is an analytical method used in chemistry that enables the identification and quantification of metabolites in samples. It differs from conventionalMR imaging in that spectra provide physiologic and chemical information instead of anatomy. MR spectroscopy and MR imaging have their origin in nuclear magnetic resonance (NMR). NMR was first described in 1946 si...