1. Organophosphorus compounds that produce a delayed neurotoxic effect in hens phosphorylate a specific site in the brain soon after administration. 2. Phosphorylation of the specific site by di-isopropyl [(32)P]phosphorofluoridate in vitro is blocked by the prior addition of phenyl phenylacetate. 3. A small proportion of the total activity of hen brain hydrolysing phenyl phenylacetate in vitro...

We describe here the purification and identification of a previously unrecognized target for organophosphorus compounds. The target, acylpeptide hydrolase, was isolated as a tritiated-diisopropylfluorophosphate-reactive protein from porcine brain and purified to homogeneity using a combination of ion-exchange and gel-filtration chromatography. Biochemical characterization and internal sequence ...

The tertiary phosphine oxides (TPO) comprise a large group of organophosphorus compounds to which a considerable attention in the literature is paid [1-5]. However, only few TPO ’s containing one or more primary amino groups in their mole­ cules are known. Studies on such compounds are limited [6-12]. Because of the high activity of amino groups these compounds can be used as phosphorylation ag...