background: adipose-derived stem cells (adsc) could be an appealing alternative to bone marrow stem cells (bmsc) for engineering cell-based osteoinductive grafts. meanwhile, prior studies have demonstrated that melatonin can stimulate osteogenic differentiation. therefore, we assayed and compared the melatonin effect on osteogenic differentiation of bmsc with that of adsc. methods: mesenchymal ...

BACKGROUND The demand for a large amount of osteogenic cells required in bone tissue engineering warranted exploration of a new source of osteoprogenitor cells. Recent studies have demonstrated that adipose-derived stromal cells possess multiple differentiation capacities, including osteogenic potential, as bone marrow mesenchymal stem cells. In the present study, the authors compared the osteo...

INTRODUCTION Bone morphogenetic proteins (BMPs) are critical growth factors in the osteogenic differentiation of progenitor cells during development in embryos and fracture repair in adults. Although recombinant BMPs are in use clinically, their clinical efficiency needs to be improved. The biological activities of BMPs are naturally regulated by extracellular binding proteins. The specific hyp...

Transforming growth factor-β (TGF-β) is a critical regulator of osteogenic differentiation and the platelet-derived growth factor (PDGF) is a chemoattractant or mitogen of osteogenic mesenchymal cells. However, the combined effects of these regulators on the osteogenic differentiation of mesenchymal cells remains unknown. In this study, we investigated the effects of TGF-β and/or PDGF on the os...

In 1935 Roberts and Roberts reported the occurrence of osteogenic sarcoma in nonidentical siblings (one brother and two sisters) with no previous chronic bone disorder. In this report fibrosarcoma and osteogenic sarcoma were included : one tumour was diagnosed as fibrosarcoma, one as sclerosing osteogenic sarcoma, and one as round-cell osteogenic sarcoma. A year later the occurrence of osteogen...

Although human umbilical cord mesenchymal stromal cells (hUCMSCs) have been shown to differentiate along an osteogenic lineage in monolayer culture, the potential of these cells has seldom before been investigated in three-dimensional scaffolds for bone tissue engineering applications. In this 6-week study, we observed osteogenic differentiation of hUCMSCs on polyglycolic acid (PGA) nonwoven me...

Although studies in vivo revealed promising results in bone regeneration after implantation of scaffolds together with osteogenic progenitor cells, basic questions remain how material surfaces control the biology of mesenchymal stem cells (MSC). We used human MSC derived from bone marrow and studied the osteogenic differentiation on calcium phosphate surfaces. In osteogenic differentiation medi...

PURPOSE Although metastasis is the primary determinant of poor survival of patients with osteogenic sarcoma, some patients live much longer than others, indicating metastatic heterogeneity underlying survival outcome. The purpose of the investigation was to identify genes underlying survival outcome of patients with osteogenic sarcoma metastasis. EXPERIMENTAL DESIGN We have used microarray to...

Background: Adipose-derived stem cells (ADSC) could be an appealing alternative to bone marrow stem cells (BMSC) for engineering cell-based osteoinductive grafts. Meanwhile, prior studies have demonstrated that melatonin can stimulate osteogenic differentiation. Therefore, we assayed and compared the melatonin effect on osteogenic differentiation of BMSC with that of ADSC. Methods: Mesenchymal...

Osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) is accurately regulated by essential transcription factors and signaling cascades. However, the precise mechanisms involved in this process remain unknown. MicroRNAs (miRNAs) regulate various biological processes by binding target mRNA to attenuate protein synthesis. The purpose of this study was to investigate the role o...