objective: to evaluate the potentiality of ovcar–3 cell line of ovarian adenocarcinoma as a xenograft model for ovarian adenocarcinoma in nude mice. materials and methods: the cell line isolated from advanced human ovarian adenocarcinoma, were inoculated to eight nude mice and two months later. established tumors were transferred to pathology laboratory to be prepared by h&e; staining and immun...

Objective(s): Extracellular matrix (ECM) is composed of many kinds of glycoproteins containing glycosaminoglycans (GAGs) moiety. The research was conducted based on the N-Acetylgalactosamine (GalNAc) degradation of ECM components by α-N-acetylgalactosaminidase (Nagalase) which facilitates migration and invasion of cancer cells. This study aims to investigate the effects of Naga-shRNA downregula...

This study examined the role of the immuno-suppressive enzyme indoleamine-2,3-dioxygenase (IDO) in ovarian cancer progression, and the possible application of this enzyme as a target for ovarian cancer therapy. We transfected a short hairpin RNA vector targeting IDO into the human ovarian cancer cell line SKOV-3, that constitutively expresses IDO and established an IDO downregulated cell line (...

background: adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines.  it has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer.  this study has managed to investigate endogenous and/or exogenous expression of visfatin and resisti...

background: mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. this study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different non-viral transfection procedures. methods: in the current study, calcium phosphate (cap), deae-...

Objective(s): For the development of reliable, ecofriendly, less expensive process for the synthesis of silver nanoparticles and to evaluate the bactericidal, and cytotoxicity properties of silver nanoparticles synthesized from root extract of Croton bonplandianum, Baill. Materials and Methods: The synthesis of silver nanoparticles by plant part of Croton bonplandianum was carried out.  The for...

BACKGROUND Ovarian cancer is the most lethal malignant tumor of the female reproductive system, and the metastasis is one of the major factors that contribute to the poor outcome of patients with OC. Accumulating evidence indicates that lncRNAs are expressed and play important regulatory roles in ovarian cancer. MATERIAL AND METHODS Aberrant lncRNAs in primary ovarian cancer tissues (POCTs) and...

background: ovarian cancer is the fifth leading cause of death from malignancy in women. cd4 +cd25+foxp3+ regulatory t (treg) cells are a subset of t lymphocytes with great inhibitory impact on immune response. objectives: to investigate the percentage of cd4 +cd25+foxp3+ regulatory t cells in the peripheral blood of the iranian patients with epithelial ovarian cancer compared to healthy women ...

background: bleomycin (blm) is an anti-cancer drug widely used in the treatment of cancer. blm causes several side effects related to dna and cellular damage. the aim of this study was investigated the effects of tea polyphenol epicatechin on anti-proliferative effects induced by bleomycin in human normal skin and human ovarian cancer cells.materials and methods: human ovarian cancer cell (skov...

BACKGROUND The role of estrogen and progesterone in ovarian carcinogenesis and the growth and survival of ovarian cancer cells is controversial. In this study, we tested the effects of various concentrations of estrogen and progesterone on the survival of an ovarian cancer cell line. METHODS The ovarian adenocarcinoma cell line, OC-117-VGH, is deficient in the following receptors: estrogen re...