نتایج جستجو برای: p. pastoris

تعداد نتایج: 1271785  

2011
Hidetsugu Asada Tomoko Uemura Takami Yurugi-Kobayashi Mitsunori Shiroishi Tatsuro Shimamura Hirokazu Tsujimoto Keisuke Ito Taishi Sugawara Takanori Nakane Norimichi Nomura Takeshi Murata Tatsuya Haga So Iwata Takuya Kobayashi

BACKGROUND Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled receptors (GPCRs) for structural studies. Recently, the crystal structures of four recombinant human GPCRs, namely β2 adrenergic receptor, aden...

Journal: :Biotechnology and applied biochemistry 1999
T Kjeldsen A F Pettersson M Hach

The yeasts Pichia pastoris and Saccharomyces cerevisiae have similar overall features regarding the secretory expression of insulin. The S. cerevisiae mating factor alpha (alpha-factor) prepro-leader facilitated the secretion of an insulin precursor, but not proinsulin expressed in P. pastoris. Synthetic prepro-leaders developed for the secretory expression of the insulin precursor in S. cerevi...

Journal: :Microbial Cell Factories 2005
Cemal Gurkan David J Ellar

The methylotrophic yeast Pichia pastoris is a popular heterologous expression host for the recombinant production of a variety of prokaryotic and eukaryotic proteins. The rapid emergence of P. pastoris as a robust heterologous expression host was facilitated by the ease with which it can be manipulated and propagated, which is comparable to that of Escherichia coli and Saccharomyces cerevisiae....

Hosnieh Ghasemi, Mahsa Nayebhashemi, Monire Jamalkhah, Mozhgan Zahmatkesh, Nafiseh Moeinian, Najmeh Zarei, Somayeh Enayati, Vahid Khalaj, Zahra Mohammadi,

Background: The methylotrophic yeast Pichia pastoris is an appealing production host for a variety of recombinant, including biologics. In this sense, various genetic- and non-genetic-based techniques have been implemented to improve the production efficiency of this expression platform. Los1 (loss of supression) encodes a non-essential nuclear tRNA exporter in Saccharomyces cerevisiae, which i...

Journal: :Journal of bioscience and bioengineering 2001
M Inan M M Meagher

The growth of Pichia pastoris in a mixture of either glycerol or glucose and methanol follows a diauxic growth, with C1 utilizing enzymes being repressed. Therefore, these carbon sources can not be used as a mixture with methanol to simultaneously grow P. pastoris and induce C1 utilizing enzymes, especially in a shake flask cultures of AOX-deficient P. pastoris. Among the alternative carbon sou...

2011
Xin Jin Nan Meng Li-ming Xia

The endo-β-1,4-glucanase gene celE from the anaerobic fungus Orpinomyces PC-2 was placed under the control of an alcohol oxidase promoter (AOX1) in the plasmid pPIC9K, and integrated into the genome of a methylotrophic yeast P. pastoris GS115 by electroporation. The strain with highest endo-β-1,4-glucanase activity was selected and designed as P. pastoris egE, and cultivated in shaking flasks. ...

2012
M. Jahic

Contents 1. Introduction 2. The P. pastoris expression system 2.1. The AOX1 promoter and alternative promoters 2.1.1 AOX1 promoter. 2.1.2. Strongly expressed alternative promoters. 2.1.3. Moderately expressed alternative promoters. 2.2. Host strains 2.2.1. Methanol utilization phenotype 2.2.2. Protease-deficient host strains 2.3. Expression vectors 3. Bioreactor process techniques for productio...

2014
Hyun Kang

Purpose: To investigate Pichia pastoris expression system for producing clinically usable, high-quality dipeptidyl peptidase 4 recombinant protein. Methods: The yeast, Pichia pastoris, expression system was used for the production of the human recombinant dipeptidyl peptidase 4 as a secreted form. The full-length human dipeptidyl peptidase 4 corresponding to the amino acid 31-766 was subcloned ...

2013
Jie-Xian Dong Xi Xie Yong-Sheng He Ross C. Beier Yuan-Ming Sun Zhen-Lin Xu Wei-Jian Wu Yu-Dong Shen Zhi-Li Xiao Li-Na Lai Hong Wang Jin-Yi Yang

A Pichia pastoris (P. pastoris) cell surface display system of Bombyx mori acetylcholinesterase (BmAChE) was constructed and its bioactivity was studied. The modified Bombyx mori acetylcholinesterase gene (bmace) was fused with the anchor protein (AGα1) from Saccharomyces cerevisiae and transformed into P. pastoris strain GS115. The recombinant strain harboring the fusion gene bmace-AGα1 was in...

Journal: :Autophagy 2007
Jean-Claude Farré Jason Vidal Suresh Subramani

The cytoplasm-to-vacuole targeting (Cvt) pathway of Saccharomyces cerevisiae delivers aminopeptidase I (Ape1) from the cytosol to the vacuole, bypassing the normal secretory route. The Cvt pathway, although well-studied, was known only in S. cerevisiae. We demonstrate its existence in the methylotrophic yeast, Pichia pastoris, where it also delivers P. pastoris Ape1 (PpApe1) to the vacuole. Mos...

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