Assembly of the heterotrimeric influenza virus polymerase complex from the individual subunits PB1, PA, and PB2 is a prerequisite for viral replication. The conserved protein-protein interaction sites have been suggested as potential drug targets. To characterize the PB1-PB2 interface, we fused the PB1-binding domain of PB2 to green fluorescent protein (PB2(1-37)-GFP) and determined its competi...

Different structural chemistries resulting from the Pb2+ lone-pair electrons in the PbMO3 perovskites are reviewed. The Pb2+ lone-pair electrons enhance the ferroelectric transition temperature in PbTiO3, stabilize vanadyl formation in PbVO3, and induce a disproportionation reaction of CrIV in PbCrO3. A Pb2+ + NiIV = Pb4+ + NiII reaction in PbNiO3 stabilizes the LiNbO3 structure at ambient pres...

Our aim was to investigate the biosorption of Pb2+, Hg2+, Cd2 from aqueous solution by Aspergillus terreus (both free and immobilized on loofa sponge discs). Our results show that the adsorption capacity of fungal biomass on loofa sponge (FBLS) is superior to free fungal biomass (FFB). The adsorption selectivity by FBLS was in the order Pb2+>Hg2+>Cd2+. The maximum metal ions adsorbed was 247.2,...

When Fe2+ ions are added to rat-liver microsomes, lipid peroxidation begins after a short lag period. Fe2+-dependent peroxidation in the first few minutes of the incubation can be increased by adding Fe3+, ascorbic acid or Pb2+ ions; these stimulations are not additive. By contrast, Pb2+ ions inhibit peroxidation of microsomes in the presence of Fe3+/ascorbate or Fe3+-ADP/NADPH. In liposomes ma...

Reaction of chromium(III) precursors with TeO2 in PbF2/PbO melts in air led to oxidation of chromium(III) to chromium(VI), whereas tellurium remained its oxidation state of IV. In the resulting title compound, Pb2(CrO4)(TeO3), the two types of anions are isolated from each other, hence a double salt is formed. The two independent Pb2+ cations exhibit coordination number nine under formation of ...

H6 subtype influenza viruses are commonly isolated from wild aquatic birds. However, limited information is available regarding H6 influenza virus isolated from chickens. We compared the viral genome segment between A/chicken/Hong Kong/W312/97 (H6N1), which was able to grow in chicken trachea, and A/duck/Shantou/5540/01 (H6N2), which was isolated from wild aquatic duck, to explore the factors f...

Lead (Pb2+) poisoning causes hypertension, but little is known regarding its acute effects on cardiac contractility. To evaluate these effects, force was measured in right ventricular strips that were contracting isometrically in 45 male Wistar rats (250-300 g) before and after the addition of increasing concentrations of lead acetate (3, 7, 10, 30, 70, 100, and 300 microM) to the bath. Changes...

The lead(II) complexes of 2,2'-iminodiethanol (H2Dea)Pb2(Dea)(N03)2(DMF), Pb2(HDea)2Cl2 and [Pb2Br6](H3Dea)2(DM F = N,N-dimethylformamide) have been synthesized from the com­ ponents and characterized by X-ray crystallography. Both mono(HDea) and doubly (Dea) deprotonated HN (CH2CH2OH)2 are coordinated via oxygen and nitrogen atoms in Pb2(D ea)(N 03)2(DM F) and Pb2(HDea)2Cl2. The nitrate comple...

Inorganic lead ion in micromolar concentrations inhibits Electrophorus electroplax microsomal (Na+ + K+)-adenosine triphosphatase ((Na+ + K+)-ATPase) and K+-p-nitrophenylphosphatase (NPPase). Under the same conditions, the same concentrations of PbCl2 that inhibit ATPase activity also stimulate the phosphorylation of electroplax microsomes in the absence of added Na+. Enzyme activity is protect...

In this study, reduced graphene oxide (rGO) was electrochemically deposited on the surface of screen-printed carbon electrodes (SPCE) to prepare a disposable sensor for fast detection of Pb2+ in foods. The SEM images showed that the rGO was homogeneously deposited onto the electrode surface with a wrinkled nanostructure, which provided 2D bridges for electron transport and a larger active area ...