Results In PC12 cells stably expressing rat P2Y1 receptors (PC12P2Y1), but not in wild type PC12 cells (PC12-wt), ADP induced rises in intracellular Ca2+ with half-maximal effects at 15 ± 1.3 μM. In whole-cell patch-clamp recordings, ADP inhibited ICa of PC12-P2Y1 cells (EC50: 6.3 ± 1.7 μM) and of PC12-wt (EC50: 3.8 ± 1.3 μM); this effect was not altered by the P2Y1 antagonist MRS 2216 (1 μM), ...

We have previously reported that 5 copies of the hypoxia response element (HRE) can conditionally regulate brain-derived neurotrophic factor gene expression under hypoxic/ischemic conditions in mice. In the present study, we investigated the controlled expression of neurotrophin-3 (NT-3) by HRE under hypoxic conditions and determined the protectiv...

The studies described here characterize abnormalities in the expression of neurofilament (NF) proteins in a clonal rat pheochromocytoma (PC12) cell line as compared with normal NF proteins in cultured rat sympathetic neurons (RSNs). Cytoskeletal extracts from PC12 cells grown in the presence (PC12+ cells) or absence (PC12- cells) of nerve growth factor (NGF) and from RSNs grown in the presence ...

Previous studies have shown that in neuronal cells the developmental phenomenon of programmed cell death is an active process, requiring synthesis of both RNA and protein. This presumably reflects a requirement for novel gene products to effect cell death. It is shown here that the death of nerve growth factor-deprived neuronal PC12 cells occurs at the same rate as that of rat sympathetic neuro...

Cells of the rat neuronal line, PC12, adhere well to substrates coated with laminin and type IV collagen, but attach poorly to fibronectin. Adhesion and neurite extension in response to these extracellular matrix proteins are inhibited by Fab fragments of an antiserum (anti-ECMR) that recognizes PC12 cell surface integrin subunits of M~ 120,000, 140,000, and 180,000 (Tomaselli, K. J., C. H. Dam...

The effects of pertussis toxin, an uncoupler of Gi protein from adenylate cyclase, and luzindole, a competitive inhibitor of melatonin receptor binding, were examined for their ability to inhibit melatonin-induced suppression of PC12 cell growth. Both agents inhibited the melatonin response suggesting that melatonin may be acting through one of its Gi coupled cell surface receptors. This is con...

Neurotrophins are required for the differentiation and survival of several different neuronal subpopulations in the developing nervous system. The PC12 cell line responds to nerve growth factor (NGF) by withdrawing from the cell cycle and acquiring a sympathetic neuron-like phenotype. Previous studies have shown that the activation kinetics of the NGF receptor, TrkA, and downstream protein kina...

objective: this study was planned to investigate whethercoriandrum sativum (c. sativum) is capable of protecting neurons against glucose/serum deprivation (gsd)-induced cytotoxicity. material and methods: the pc12 cells were cultivated for 24 h in standard media (high-glucose dmem containing fetal bovine serum) or for 6 h in gsd condition (glucose-free dmem, without serum) in the absence or pre...