PURPOSE To validate the use of polymerase chain reaction (PCR)-amplified full-length cDNA as a substitute for mRNA in nucleic acid array and gene expression analysis. METHODS Total RNA was isolated from age-matched normal autopsy corneas and pseudophakic bullous keratopathy (PBK) corneas. Full-length cDNA was generated and PCR amplified using the Smart cDNA synthesis technology. Southern blot...

Genomic instability is a classic hallmark of cancer and genetic disorders. The Agilent Oligonucleotide array-based Comparative Genomic Hybridization (aCGH) platform lets you profile DNA copy number variations on a highthroughput and genome-wide scale. We have developed a rapid, low-input whole genome amplification (WGA) method for aCGH analysis that pairs the Sigma GenomePlex WGA Kit with Agile...

Cystic fibrosis (CF) is the most common inherited genetic disease in Caucasian populations. Besides bacteria, many species of fungi may colonize the respiratory tract of these patients, sometimes leading to true respiratory infections. In this study, an oligonucleotide array capable of identifying 20 fungal species was developed to directly detect fungi in the sputum samples of CF patients. Spe...

Uniform linear array (ULA)-based tapped-delay line (TDL) structure has good performance metrics when the signal sources are located at the middle angles. It offers poor performance when the signal sources are close to the array endfire. In this paper, a new approach is proposed which offers higher performance and desired beamforming on TDL structure when the wideband uncorrelated radio sources ...

In this paper, we show an integrated complementary metal oxide semiconductor (CMOS)-based microchip system with capillary array electrophoresis (CAE) for the detection of bacterial pathogen amplified by polymerase chain reaction (PCR). In order to demonstrate the efficacy of PCR reaction for the heat-labile toxin producing enterotoxigenic Escherichia coli (E. coli), which causes cholera-like di...

Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genotyping. One such method is reported here, named tetra-primer ARMS-PCR, which employs two primer pa...

Supplemental Method Targeted sequence capture and NGS 6 μg of genomic DNA was randomly fragmented with sizes mainly distributed between 250 and 300 bp. Adapters were ligated to both ends of the resulting fragments. DNA was then amplified by ligation-mediated PCR (LM-PCR), purified, and hybridized to the RDs189 array for enrichment, and non-hybridized fragments were then washed out. Both non-cap...

gamma camera is an important apparatus in nuclear medicine imaging. its detection part is consists of a scintillation detector with aheavy collimator. substitution of semiconductor detectors instead of scintillator in these cameras has been effectively studied. in this study, it is aimed to introduce a new design of p-n semiconductor detector array for nuclear medicine imaging. a p-n semiconduc...

A real-time secretion monitoring assay from living single-cells was realized by total internal reflection fluorescence imaging with an optically optimized microwell array chip fabricated of an amorphous fluoropolymer, CYTOP on the basis of a fluorescence sandwich immunoassay. After individual mast cells were stimulated with lipopolysaccharide in the microwell array, their proinflammatory respon...

BACKGROUND Onychomycosis is a fungal infection of nails, leading to the gradual destruction of the nail plate. Treatment of onychomycosis may need long-time oral antifungal therapy that can have potential side effects, thus accurate diagnosis of the disease before treatment is important. Culture for diagnosis of onychomycosis is time-consuming and has high false-negative rates. To expedite the ...