نتایج جستجو برای: pcr array
تعداد نتایج: 304604 فیلتر نتایج به سال:
Expanding applications of cDNA microarrays such as fine needle aspiration biopsy and laser capture microdissection necessitate the ability to perform arrays with minute starting amounts of RNA. While methods for amplifying RNA have been advocated, the fidelity of array results using amplified material has not been fully validated. Here we demonstrate preserved fidelity in arrays using one or tw...
Using Streptococcus pyogenes as a model, we previously established a stepwise computational workflow to effectively identify species-specific DNA signatures that could be used as PCR primer sets to detect target bacteria with high specificity and sensitivity. In this study, we extended the workflow for the rapid development of PCR assays targeting Enterococcus faecalis, Enterococcus faecium, Cl...
For the production of DNA microarrays from PCR products, purification of the the DNA fragments prior to spotting is a major expense in cost and time. Also, a considerable amount of material is lost during this process and contamination might occur. Here, a protocol is presented that permits the manufacture of microarrays from unpurified PCR products on aminated surfaces such as glass slides coa...
let $a_1$, $a_2$ be unital banach algebras and $x$ be an $a_1$-$a_2$- module. applying the concept of module maps, (inner) modulegeneralized derivations and generalized first cohomology groups, wepresent several results concerning the relations between modulegeneralized derivations from $a_i$ into the dual space $a^*_i$ (for$i=1,2$) and such derivations from the triangular banach algebraof t...
Ensuring mycoplasma-free cell culture is of prime importance as they severely affect cellular characteristics leading to experimental artefacts and spurious results. Various methods persist for mycoplasma detection; out of the whole array of methods polymerase chain reaction (PCR) is the most favoured one because it is highly sensitive, specific and quick. The PCR-based detection procedure invo...
Partial digestion of DNA fragments is a standard procedure for subcloning analysis and for generating restriction maps. We have developed a novel method to generate a partial digestion for any DNA fragment that can be amplified by PCR. The method involves the incorporation of 5-methyl-dCTP into the PCR product to protect most of the restriction sites. As a result, complete digestion of the modi...
چکید ه سابقه و هدف برقراری هموستاز و کنترل خونریزی ، از اصول شناخته شده در انجام انواع جراحی ها است. در برخی موارد، کنترل خونریزی با روش های معمول مانند کوتر کردن یا لیگاتور، سخت و یا غیر ممکن است. با در نظر گرفتن خواص مختلف کلاژن شامل اثر تسریع کننده بر هموستاز، جذب کامل، کمک به ترمیم بافتی، قابلیت انعطاف و کشش مناسب، می توان با استفاده از این ماده به برقراری هموستاز کمک کرد. مواد وروش ه...
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