How many intergenically encoded non-coding RNAs (ncRNAs) are expressed during various developmental stages in Drosophila? Previous analyses in one or a few developmental stages indicated abundant expression of intergenic ncRNAs. However, some reported that ncRNAs have been recently falsified, and, in general, the false positive rate for ncRNA detection is unknown. In this report, we used revers...

To facilitate the study of the mechanisms of breast cancer metastasis we have previously characterized a pair of breast tumor cell lines that originate from the same breast tumor cell line MDA-MB-435, but which have diametrically opposite metastatic capabilities. These cell lines constitute a stable and accessible experimental system for the identification of metastasis-related genes and for th...

Sulfur mustard (SM) is a strong alkylating and mutagenic compound that targets humanairway system. We considered the expression of Forkhead box M1 (FOXM1) and apolipoproteinE (APOE) genes, which are responsible for cell proliferation, differentiation, tumorigenesis,and increased risk of lung cancer, in the lung bronchial tissue of patients exposed to SM.After performing pulmonary functional tes...

Sulfur mustard (SM) is a strong alkylating and mutagenic compound that targets humanairway system. We considered the expression of Forkhead box M1 (FOXM1) and apolipoproteinE (APOE) genes, which are responsible for cell proliferation, differentiation, tumorigenesis,and increased risk of lung cancer, in the lung bronchial tissue of patients exposed to SM.After performing pulmonary functional tes...

Abstract Introduction/Objective Introduction: Standard diagnostic practices for Urinary Tract Infection (UTI) take 2–3 days pathogen identification and antibiotic susceptibility testing leading to delayed diagnosis an increase in healthcare expenditures. The rapid diagnostics tool is highly desirable identify the its antibiotic-resistant genes directly from samples. Our objective validate evalu...

Detection of KRAS mutations in archival pathology samples is critical for therapeutic appropriateness of anti-EGFR monoclonal antibodies in colorectal cancer. We compared the sensitivity, specificity, and accuracy of Sanger sequencing, ARMS-Scorpion (TheraScreen®) real-time polymerase chain reaction (PCR), pyrosequencing, chip array hybridization, and 454 next-generation sequencing to assess KR...