conclusions with regard to the ease of urine sample preparation and the 100% specificity the pcr method, performing urine pcr could be used as a diagnostic aid in ptb cases obtaining sputum samples is problematic. results results of the current study indicated that 48 (62.3%) patients out of 77 had a positive sputum culture. urine cultures and acid-fast smears were negative. urine pcr-tb was po...

background cervical cancer is the leading cause of death from cancer in under-developed countries. human papilloma virus (hpv) 16 and 18 are the most prevalent types associated with carcinogenesis in the cervix. conventional polymerase chain reaction (pcr), type-specific and consensus primer-based pcr followed by sequencing, restriction fragment length polymorphism (rflp) or hybridization by sp...

conclusions obtained data demonstrated that the designed assay is an accurate, easy, rapid, and cost-effective tool for detecting v. cholerae that can serve as an alternate to current bacteriological tests. background several epidemic and endemic cases have been reported involving vibrio cholerae (v. cholerae) as a causative organism in serious diarrheal diseases with high mortality. hence, qui...

background infections caused by gram negative bacteria, producing extended-spectrum β-lactamase (esbl), including klebsiella pneumoniae are increasing all over the world with high morbidity and mortality. objectives the aim of this study was to determine the prevalence of k. pneumoniae encoding the ctx-m, tem-1 and shv-1 esbl enzymes genes, isolated from clinical specimens. materials and method...

conclusions all the bacterial isolates were associated with a high resistance to various antibiotics and this resistant pattern could be confirmed by detection of a particular gene for each bacterium. objectives this study examined bacteremia in cancer patients from two medical centers regarding isolates and spectrum of antibiotic resistance pattern. patients and methods this was a prospective ...

human papillomavirus (hpv) has also been suggested as an etiology of esophageal squamous cell carcinoma (scc). the aim of this study was to investigate the prevalence of hpv infection in esophageal sccs in our region with strict contamination control to prevent false positive results. thirty cases of esophageal squamous cell carcinomas were chosen by simple random selection in a period of two y...

background: hepatitis b virus (hbv) causes acute and chronic liver disease worldwide. hbv has eight genotypes (a to h) which is the reflection of its genome with their characteristic geographical distribution. each genotype could have different pathogenic and therapeutic characteristics. there have been few records on hbv genotyping in general population from our region. this study aimed to det...

conclusions this study showed that cccdna can be detected in the plasma specimen of 17.9% of iranian treatment-naive patients with chronic hepatitis b infection. therefore, designing prospective studies focusing on the detection of cccdna in these patients would provide more information. results the mean (sd) age of the patients was 41.1 ± 12.4 years (range, 20 - 62 years). from a total of 106 ...

escherichia coli o157:h7 is an important human pathogen causing haemorrhagic colitis, haemolyticuraemic syndrom and thrombotic thrombocytopenic purpura. in this study, 100 ground beef samples were collected randomly from beef markets in june 2004. for isolation of the bacteria, samples were firstly enriched in modified trypticase soy broth, followed by plating onto sorbitol macconkey agar suppl...