results our study showed that the sensitivity and specificity of taqman real time pcr was 96% and 95% respectively. these values were 89% and 90, 38% and 100%, 6% and 100% for pcr, culture and microscopy, respectively. conclusions our study showed that sensitivity of taqman real time pcr was higher that pcr, culture and microscopy but specificity of culture and microscopy was more than pcr and ...

A minute of silence. Kary Banks Mullis has died (08/07/2019). was an American Biochemist and Doctor Chemistry who developed the Polymerase Chain Reaction (PCR) in 1985, considered one most important legacies history Humanity, along with description DNA (Watson, Crick Franklin) theory Relativity (Einstein). Currently, I consider anyone does not know PCR by to be illiterate, since it is a molecul...

background human papillomavirus (hpv) is associated with various benign and malignant lesions including genital condyloma and anogenital cancer. the presence of hpv-dna was studied in archival biopsies of high- and intermediate-risk lesions for cervical carcinoma in women referred to mirza hospital in tehran. patients and methods totally, 105 iranian archived biopsies collected during february ...

Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene expression studies. Since its introduction (...

was awarded the Nobel prize in Chemistry along with Michael Smith for his work on PCR2. The PCR is commonly carried out in a reaction volume of 10-200 μl in small reaction tubes (0.2-0.5 ml volumes) in a thermal cycler. The thermal cycler heats and cools the reaction tubes to achieve the temperatures required at each step of the reaction. Many modern thermal cyclers make use of the Peltier effe...