The production of informative random amplified polymorphic DNA (RAPD) markers using PCR and a single primer is often accompanied by the generation of artifactual (noninformative) bands as well. When RAPD data are used to compute genetic similarity coefficients, these artifacts (false positives, false negatives, or both) can cause large biases in the numerical values of the coefficients. As a re...


 Cichlids are among the economically important which serve as a source of food for people ‎around world. A deep understanding population structure and genetic diversity ‎cichlids vital initiation conservation policies sustainable aquaculture. There is ‎paucity information on patterns variations within cichlids in ‎North-central Nigeria. This study, therefore, investigated ‎Coptodon zillii...

In the present work, strain-specific PCR primers for Lactobacillus rhamnosus Lc 1/3 are described. The randomly amplified polymorphic DNA (RAPD) technique was used to produce potential strain-specific markers. They were screened for specificity by hybridization with DNA from 11 L. rhamnosus strains. A 613-bp RAPD marker found to be strain-specific was sequenced, and a primer pair specific to L....

Using the random amplified polymorphic DNA (RAPD) technique and exploiting the unique genetics of Tetrahymena thermophila, we have identified and characterized 40 DNA polymorphisms occurring between two inbred strains (B and C3) of this ciliated protozoan. These RAPD markers permit the PCR amplification of a DNA species using template DNA from SB1969 (B strain) but fail to do so using DNA from ...

Techniques such as arbitrarily primed polymerase chain reaction (APPCR) (11), random amplified polymorphic DNA (RAPD) (12) and DNA amplification fingerprinting (DAF) (7) are powerful tools for genetic mapping, taxonomic and phylogenetic investigations and for the detection of various types of DNA damage and mutation (2,3). Nevertheless, the arbitrary nature of the DNA polymerization catalyzed i...

BACKGROUND & OBJECTIVES Infections due to seafood associated Salmonella serovars are great risk to public health. Different phenotypic characteristics have been used previously for epidemiological investigation of Salmonella. Beyond the phenotypic characterization, a reliable genetic level discriminatory method is required. Therefore, this study was attempted to use different phenotypic and mol...

In this work, we described a new approach for the isolation of a species-specific probe for the Eimeria media parasite of the rabbit based on the use of the random amplified polymorphic DNA (RAPD) technique. A specific fragment of 800 bp of the studied species was isolated after RAPD and then cloned and DIG-radiolabeled. After dot-blotting, we observed that this probe was specific for E. media....