Adenoviral vectors are now being explored as vaccine carriers to prevent infectious diseases in humans and animals. There are two strategies aimed at the expression of a vaccine antigen by adenoviral vectors. The first includes an insertion of the foreign gene expression cassette into the E1 region. The second strategy is antigen incorporation into the viral capsid proteins. To extend this meth...

A multiple antigen peptide (MAP) malaria vaccine containing minimal Plasmodium falciparum circumsporozoite protein repeat epitopes was assessed for safety and immunogenicity in volunteers of known class II genotypes. The MAP/alum/QS-21 vaccine formulation elicited high levels of parasite-specific antibodies in 10 of 12 volunteers expressing DQB1*0603, DRB1*0401, or DRB1*1101 class II molecules....

Currently, infections of hand, foot and mouth disease (HFMD) due to Human Enterovirus 71 (EV71) cannot be prevented or treated, as there are no suitable vaccines or antiviral drugs. This study aimed to identify potential vaccine candidates for EV71 using in silico analysis of its viral capsid proteins. A combined in silico approach utilizing computational hidden Markov model (HMM), propensity s...

Introduction: Cancer vaccines have the potential to induce curative anti-tumor immune responses and better adjuvants may improve vaccine efficacy. We have previously shown that Hp91, a peptide derived from the B box domain in high-mobility group box protein 1 (HMGB1), acts as a potent immune adjuvant. Method: In this study, Hp91 was tested as part of a therapeutic vaccine against human epiderma...

This study aimed to perform the analysis of toxicity prediction, allergenicity and in silico cloningof peptides originated from SARS-CoV-2 spike glycoprotein previous study. Allergenicity predictionemployed AllerPred predict non-allergen prediction performed using ToxinPred.Then, this designed cloning with pET-28a(+) usingSnapGene software. Therefore, successfully constructed vaccine candidate ...

BACKGROUND & OBJECTIVES Most group A streptococcal (GAS) vaccine strategies focused on the surface M protein of the GAS. However, vaccine based on M protein have some drawbacks. In the present study, we used two approaches to identify new proteins and peptides that may have utility as vaccine candidates. METHODS A whole gel elution procedure was used to separate GAS surface antigens into 9 si...

Epitope peptides are not suitable for nasal administration immunity due to their poor immunogenicity and low delivery efficiency. Here, we reported an intranasal self-assembled nanovaccine (I-OVA NE), which was loaded with the IKVAV-OVA257–264 (I-OVA), a laminin peptide (Ile-Lys-Val-ala-Val, IKVAV) OVA257–264 epitope conjugated peptide. This I-OVA at concentration of 4 mg/mL showed average part...

These are exciting times in the development of new immunotherapeutic strategies for cancer therapy. In particular, synthetic peptide-based vaccines, aiming at the induction of T-cell mediated anti-tumor immunity, hold immense promise for prevention and therapy of malignant diseases. Synthetic peptides are easily produced, are chemically stable, easily deliverable, free of infectious contaminant...

Dendritic cells (DCs) are potent antigen-presenting cells and have shown promise to function as "natural" vaccine adjuvants. Currently, most cancer vaccine trials using DCs generate autologous DCs ex vivo for each patient. Systemic treatment with Flt3 ligand (FL) results in a marked increase of DCs in tissues such as spleen and lymph nodes in mice and in the peripheral blood and skin of humans....

conclusions hcv gpe2 is an ideal target for hcv vaccine. prediction of epitope immunogenicity and characterization on the basis of peptide sequences will be significantly helpful for development of a heterologous vaccine against hcv variants. results many b-cell and t-cell epitopes predicted in gpe2 were found conserved among hcv 3a genotypes whereas few were conserved in other genotypes antici...