Serum gastrin and gastric acid responses to a test meal of 10% peptone were measured in six duodenal ulcer patients using intragastric titration at pH levels ranging from 5.5 to 1.0. In this way the pH profile for inhibition of serum gastrin release and gastric acid secretion was established. A peptone meal adjusted to pH 5.5 produced gastric acid similar to the maximal response to histamine. A...

A simplified technique to detect polyphenol oxidase and melanin formation by Streptomyces culture filtrates was developed. The procedure involves the direct assay of pigment formation by the culture filtrate with 3-(3,4-dihydroxyphenyl)-L-alanine (L-dopa) as a substrate. Among cultures of the International Streptomyces Project, 34 failed to produce a diffusible dark brown pigment on peptone-yea...

Isolation and identification of thermophilic Bacillus sp was carried out from a soil sample. The cells were cultivated in a medium containing soluble starch as sole carbon source. The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the medium shortened the lag period and improved the growth and amylase synthesis. The optimum temperature for amylase production was detecte...

The alkaline protease gene, Apr, from Bacillus licheniformis 2709 was cloned into an expression vector pET 28b(+). The pET-28b(+) Apr was expressed in a high expression strain E. coli BL21. In order to develop and optimize mathematical model of protease activity, three variables were investigated, including peptone concentration (6 g/L to 10 g/L), incubation time (16 h to 32 h) and agitation fr...

The present study conducted a genetic characterization and determined growth rate and biomass production in solid and liquid media, using strains obtained from wild edible sporomes of Lyophyllum that grow in high mountains. Vegetative isolation was used to obtain a total of four strains, which were divided into two clades within the section Difformia: Lyophyllum sp. and Lyophyllum aff. shimeji....

Statistical analyses were applied to optimize the medium composition for the mycelial growth and polysaccharide production by Tremella aurantialba in shake flask cultures. Firstly, four significant factors (xylan, peptone, wheat bran and corn powder) on mycelial growth and polysaccharide yield (p£0.05) were obtained using one-at-a-time design. Subsequently, in order to study the mutual interact...

The cellulase activity of Bacillus subtilis AS3 was enhanced by optimizing the medium composition by statistical methods. The enzyme activity with unoptimised medium with carboxymethylcellulose (CMC) was 0.07 U/mL and that was significantly enhanced by CMC, peptone, and yeast extract using Placket-Burman design. The combined effects of these nutrients on cellulase activity were studied using 2(...

We studied acid secretory responses to exogenous pentagastrin and to exogenous and endogenous gastrin in 12 stable cirrhotic subjects with portacaval shunt, 12 unshunted cirrhotics, and 12 normal subjects. Basal and stimulated serum gastrin concentrations as well as basal and maximum acid outputs were similar in the three groups. At low doses of either exogenous pentagastrin or gastrin-17 (G17)...