The effects of culture conditions and chloramphenicol treatment on the induction of the marine bacterium Pseudoalteromonas spongiae to larval settlement of Hydroides elegans were investigated. The results showed that P. spongiae cells grown in the medium containing both yeast extract and peptone (YP-grown P. spongiae) was highly inductive to larval settlement, whereas P. spongiae cells grown in...

The influence of treatment temperature and pulsed electric fields (PEF) on the viability of Mycobacterium paratuberculosis cells suspended in 0.1% (wt/vol) peptone water and in sterilized cow's milk was assessed by direct viable counts and by transmission electron microscopy (TEM). PEF treatment at 50 degrees C (2,500 pulses at 30 kV/cm) reduced the level of viable M. paratuberculosis cells by ...

Some years ago it was found that Bacillus subtilis sporulated more profusely in Bacto-peptone broth when a small quantity of activated charcoal was added to the medium. Further studies revealed that although the charcoal was removed from the medium prior to inoculation, the percentage of cells sporulating during short incubation periods was still appreciably greater than in untreated Bacto-pept...

The synthesis of an extracellular protease by Bacillus sp. HTS102—a wild strain recently isolated from the wool of Portuguese Merino ewes, was optimized. This protease is thermostable and particularly resistant to harsh environmental conditions—and appears to bear a unique ability to hydrolyze keratin-rich solid materials. Following a preliminary screening for the most relevant medium factors i...

1. Many substances besides the specific broth filtrates of Besredka can be utilized as topical applications to protect guinea pigs from the effects of massive doses of staphylococcus given subcutaneously. (Plain broth, peptone 10 per cent, peptone 1 per cent, Liebig's meat extract, mustard plaster and normal horse serum.) 2. Where such protection occurs, no matter what the stimulus is, the loca...

A statistical design approach has been used to optimize the production of biomass by Cunninghamella sp. 2A1, evaluated based on lipidless biomass. A 2 3 full factorial central composite design (CCD) was chosen to study the combined effects of three factors; ammonium tartrate, peptone and glucose concentrations. The p-value for each factor was <0.05 suggesting that these factors have significant...

effect on T lymphocytes. Fermentations for the production of conagenin were done as follows. S. roseosporus was cultured in 500-ml Erlenmeyer flasks containing 1 10 ml of the autoclaved seed medium consisting of galactose 2.0%, dextrin 2.0%, soy peptone 1.0%, corn steep liquor 0.5%, (NH4)2SO4 0.2%, CaCO3 0.2% and one drop of silicone. The seed medium was adjusted to pH 7.4 with 1 n HC1 and was ...

Different endophytes isolated from the seeds of Sophora flavescens were tested for their ability to produce matrine production. Response surface methodology (RSM) was applied to optimize the medium components for the endophytic fungus. Results indicated that endophyte Aspergillus terreus had the ability to produce matrine. The single factor tests demonstrated that potato starch was the best car...

On former occasions we have discussed repeatedly the action of tissue extract and serum separately, as well as their combined action on fluoride, hirudin, and peptone plasma of vertebrate blood,l We pointed out the loss in power of a mixture of serum and tissue extract on standing and the difference in the behavior of fluoride plasma (and similar plasmas) in which coagulation had been prevented...

The present study is concerned with the improved invertase production by Saccharomyces cerevisiae on kinetic basis. The wild-culture (IIB-1) and putative mutant (NA6) of S. cerevisiae were compared on time course basis when grown in batch culture. The values for Yx/s, Yp/s and Yp/x with urea were significantly improved (p≤0.05) over the control (peptone+yeast extract) as well as peptone supplem...