Denaturing high-performance liquid chromatography (DHPLC) compares two or more chromosomes as a mixture of denatured and reannealed PCR amplicons, revealing the presence of a mutation by the differential retention of homo- and heteroduplex DNA on reversed-phase chromatography supports under partial denaturation. Temperature determines sensitivity, and its optimum can be predicted by computation...

background ziziphora tenuior l. known as kakuti in persian, is used in traditional medicine for fever, dysentery, uterus infection and as an analgesic. it is used also in the treatment of gastrointestinal disorders as carminative, or remedy of diarrhea or nausea. major components of plant essential oil including pulegone, isomenthone, thymol, menthone, and piperitone are suggested to be respons...

background valproic acid is widely used as an anticonvulsant and mood-stabilizing drug in the treatment of epilepsy. determination of serum valproic acid is required in epilepsy therapy for efficient control of seizures. objectives in the present study, the gas chromatography method was applied to determine serum valproic acid and resulting data were compared with those obtained by high perform...

We describe a "high-performance" liquid-chromatographic procedure for determination of creatinine in serum. Creatinine is separated from other species by cation-exchange chromatography. Absorbance of the creatinine in the eluate is monitored at 234 nm. Results from this procedure on sera correspond closely to those of a manual Jaffé-reaction procedure involving use of a protein-free filtrate wi...

mass spectrometry method is developed and validated for the simultaneous quantitation of three major phenolic acids including 1,5-dicaffeoylquinic acid (1,5-DCQA), 3,4-dicaffeoylquinic acid (3,4DCQA), and 3,5-dicaffeoylquinic acid (3,5-DCQA) in rat plasma. All analytes and internal standard (bergeninum) are extracted from plasma samples by liquid–liquid extraction with isopropanol. The chromato...

Modern chromatography by virtue offantastic capabilities for separation, qharacterization, quantitation and fractionation has emerged as a potential analytical technique for hydrocarbon industry. Presently the gas chromatography alongwith high performance liquid chromatography coverwide range ofapplications in diversified areas including Basic and applied research. Availability of large number ...

A simple and efficient method, ionic liquid-based dispersive liquid-liquid microextraction combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV), has been applied for the extraction and determination of some antioxidants (Irganox 1010, Irganox 1076 and Irgafos 168) in water samples. The microextraction efficiency factors were investigated and optimized: 1-hexyl-3-...