mitochondria play key roles in many cell functions including energy production, fatty acid metabolism, pyrimidine biosynthesis, calcium homeostasis, and aging. they also regulate crucial signaling cascades such as apoptosis and oxidative stress. the proteome is often used to investigate the functional correlations on protein levels. based upon the human, genome there is estimated 2000 to 2500 a...

the aim of this study was to investigate the association of bovine leukocyte antigen-drb3 alleles with peripheral blood mononuclear cell (pbmc) proliferation in response to staphylococcus aureus. the animals included in this study (n=347) were approximately of same age and comprised of f2 holstein-friesian ´ charolais (n = 155), holstein-friesian backcross (f0 holstein-friesian dams crossed wit...

Abstract Clinical trials of systemic T cell checkpoint blockade in GBM patients showed only disappointing results. This may be attributed part to the immunosuppressive components immune tumor microenvironment (iTME). Therefore, major efforts have been undertaken describe iTME on a single level. However, human data composition different regions (contrast enhancing center versus peripheral infilt...

Background: Several biological and medical benefits of Saffron, Crocus sativus (Iridaceae), have been demonstrated. However, mechanisms of actions for purified constituents are greatly unknown. Objective: To examine the effects of Safranal, a main constituent of Saffron stigma, on cell viability and cytokine profile of peripheral blood mononuclear cells (PBMC) were examined. Methods: Effect...

Abstract Background Kawasaki disease (KD) is the main cause of acquired heart in children and can lead to coronary artery lesions. This present study was designed analyze characteristics KD peripheral blood mononuclear cells (PBMC) through single-cell RNA sequencing (scRNA-seq) explore potential molecular mechanism KD. Methods PBMC collected from one healthy child patient, used for cell cluster...

Abstract Background Clinical trials of systemic T cell checkpoint blockade in GBM patients showed only disappointing results. This may be attributed part to the immunosuppressive components immune tumor microenvironment (iTME). Therefore, major efforts have been undertaken describe iTME on a single level. However, human data composition different regions (contrast enhancing center versus periph...

Medicinal mushrooms, Coriolus versicolor and Lentinus edodes are extremely attractive as nutraceuticals. Here we used fruiting bodies to prepare novel kombucha beverage. Microbiological, physicochemical chemical properties were monitored for eleven days, while the immunological of polysaccharide extracts determined in peripheral blood mononuclear cell (PBMC) cultures. FTIR analysis showed domin...

Substance P (SP) has been implicated in peripheral and mucosal neuroimmunoregulation. However, confusion remains regarding immunocyte expression of the receptor for SP, neurokinin-1 receptor (NK-1R), and whether there is differential NK-1R expression in the mucosal versus the peripheral immune system. In the same assay systems, we examined the expression of NK-1R in human lamina propria mononuc...