currently, hematopoietic stem cell transplantation (hsct) is the only curative option for patients with beta-thalassemia major, but liver iron overload in these patients will not decrease and hepatic fibrosis may still progress despite successful hsct. liver biopsy samples were taken from 14 patients (out of 25 patients) who underwent hsct. all patients met three criteria: negative hcv antibody...

Ring sideroblasts Ring sideroblasts are erythroblasts with iron-loaded mitochondria visualized by Prussian blue staining (Perls’ reaction) as a perinuclear ring of blue granules (Figures 1D and 2C). The International Working Group on Morphology of Myelodysplastic Syndrome (IWGM-MDS) recommended that ring sideroblasts be defined as erythroblasts in which there are a minimum of five siderotic gra...

The paper by Thulborn et al. [1] on the distribution of ferritin and hemosiderin in cerebral hemorrhage implies that Perls Prussian blue stains hemosiderin and does not stain ferritin. This was most surprising to us, as we had just obtained excellent Perls stains of ferritincontaining regions of the brain [2] (Fig . 1 ). We think that given the current interest in iron in the brain and its effe...

Results The three slides of the serial sectioned material-peroxidase uncounterstained, Perls' Prussian blue, and the peroxidase counterstained with Perls' Prus-sian blue-were carefully compared by matching the architecture of the fields using a comparison bridge. Neither the prolactin nor the haemosiderin components seemed to be diminished as a result of the extra staining procedure. Low backgr...

OBJECTIVE To investigate whether iron is involved in the pathogenesis of age-related macular degeneration (AMD). METHODS Postmortem AMD-affected (nonexudative or exudative) and healthy maculas were studied using the 3,3'-diaminobenzidine-enhanced Perls Prussian blue stain. The Perls Prussian blue stain was quantified by computer-assisted analysis of digital images. To determine whether the ir...

The aim of the study was to develop a method for modification human monocytes/macrophages by iron oxide magnetic nanoparticles in vitro . Materials and methods Iron were obtained co-precipitation coated with thin SiO 2 layer polyethylene glycol 3000. Murine macrophage-like cell line RAW 264.7, primary monocytes macrophages incubated 1–24 hours. efficiency cellular uptake measured using ferrozin...

conjugation of monoclonal antibodies to super paramagnetic nanoparticles is an effective method for cancer diagnosis and treatment. in this study the humanized anti her2/neu monoclonal antibody- herceptin- was conjugated to super paramagnetic iron oxide (spio) nanoparticles using edc method. the concentration of the conjugated antibodies was measured by bradford assay. the antibody-nanoparticle...

Fig. 1. (A) Peripheral blood shows neutrophils with toxic granules and two intra-cytoplasmic yeast forms of Histoplasma capsulatum in the cell on the left (May-Grunwald Giemsa, ×1,000). Inset: The fungus was brightly positive for periodic acid–Schiff stain (hematoxylin counterstain, ×1,000). (B) Hemodilute bone marrow smears showed dysgranulopoiesis with 5% blasts (May-Grunwald Giemsa, ×1,000)....

The aim of the present study was to observe the in vivo targeting characteristic of angiopoietin 2-small interfering RNA (Ang2-siRNA) plasmid/chitosan magnetic nanoparticles in an established nude mouse model of malignant melanoma (MM) under an external magnetic field. The nude mouse MM model was first established, then divided into 3 groups, including the control group, the non-targeting group...

Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes [1]. Hematoxylin and eosin stain (H&E stain or HE stain), is the routine staining for skin biopsies. However, in some circumstances, d...