INTRODUCTION Reducing the amount of testing required to accurately detect cognitive impairment is clinically relevant. The aim of this research was to determine the fewest number of clinical measures required to accurately classify participants as healthy older adult, mild cognitive impairment (MCI), or dementia using a suite of classification techniques. METHOD Two variable selection machine...

To gain insight into the mechanisms that regulate the development of the H chain CDR3 (CDR-H3), we used the scheme of Hardy to sort mouse bone marrow B lineage cells into progenitor, immature, and mature B cell fractions, and then performed sequence analysis on V(H)7183-containing Cmu transcripts. The essential architecture of the CDR-H3 repertoire observed in the mature B cell fraction F was a...

Deoxycytidine kinase, which phosphorylates deoxycytidine (CdR) and its analog, cytosine arabinoside (ara-C), has been purified 71-fold from human leukemic cells. Biochemical properties of the partially purified enzyme included a molecular weight of 68,000, Kms of 7.8 muM for CdR and 25.6 muM for ara-C, and optimal activity with ATP and GTP as phosphate donors. Ara-C phosphorylation was strongly...

A problem with CDR is that it disregards the age structure of the population. For this reason, it is called crude. If people in an area are predominantly old, a high CDR is not as bad as in an area where the population is predominantly young. Thus, a CDR of 7 per 1000 population in the year 20010 in Sweden should not be construed to mean that the health status is nearly the same as in India, wh...

Background: Mammalian cell division is regulated by a complex includes cyclin-dependent kinases (Cdks) and cyclins, Cdk/cyclin complex. The activity of the complex is regulated by Cdk inhibitors (CKIs) compressing CDK4 (INK4) and CDK-interacting protein/kinase inhibitory protein (CIP/KIP) family. Hypermethylation of CKIs has been reported in various cancers. DNA methyltransferase inhibitors (DN...

To characterize and compare the thymidine (TdR) and deoxycytidine (CdR) kinase isozymes of uninfected and herpesvirus-infected cells: (i) the subcellular distribution of the isozymes has been studied; (ii) a specific assay for CdR kinase has been devised; (iii) the TdR kinase isozymes have been partially purified; and (iv) the purified enzymes have been analyzed by disc polyacrylamide gel elect...

5-Azacytidine- and 5-aza-deoxycytidine (5-aza-CdR)-mediated reactivation of tumor suppressor genes silenced by promoter methylation has provided an alternate approach in cancer therapy. Despite the importance of epigenetic therapy, the mechanism of action of DNA-hypomethylating agents in vivo has not been completely elucidated. Here we report that among three functional DNA methyltransferases (...

OF THE DISSERTATION Identification of Novel Fluid Biomarkers for Alzheimer’s Disease by Rebecca June Craig-Schapiro Doctor of Philosophy in Biology and Biomedical Sciences Neurosciences Program Washington University in St. Louis, 2012 Dr. David M. Holtzman, Chairperson Clinicopathological studies suggest that Alzheimer’s disease (AD) pathology begins to appear ~10-20 years before the resulting ...

BACKGROUND/AIMS Measuring and predicting Alzheimer's disease (AD) progression is important in order to adjust treatment and allocate care resources. We aimed to identify a combination of subtests from the Consortium to Establish a Registry for Alzheimer's Disease Neuropsychological Battery (CERAD-NB) that best correlated with AD progression in follow-up as well as to predict AD progression. M...

The effect of doses of mixed gamma-neutron radiations or 14 MeV neutrons on the urinary excretion of deoxycytidine (CdR) was evaluated as a biodosimeter in rats. The rats were exposed to doses in the range from 22 to 780 rads. The excretion of CdR in the urine of irradiated rats during the 4to 12-hour postirradiation interval was a function of dose over the range of 22 to 376 radsThis response ...