An anti-petunia glycine-rich protein 1 (ptGRP1) antibody was used for biotin-streptavidin-alkaline phosphatase localization of this protein. In petunia stem and leaves grown under different light conditions, these studies revealed a complex pattern of cell localization for this protein. Levels of ptGRP1 were shown to decrease with developmental age of the tissue, appearing to correlate directly...

The expansins comprise a family of proteins that appear to be involved in the disruption of the noncovalent bonds between cellulose microfibrils and cross-linking glycans, thereby promoting wall creep. To understand better the expansion process in Petunia hybrida (petunia) flowers, we isolated a cDNA corresponding to the PhEXP1 alpha-expansin gene of P. hybrida. Evaluation of the tissue specifi...

A method for the recovery of full-length cDNAs from predicted terpene synthase genes containing introns is described. The approach utilizes Agrobacterium-mediated transient expression coupled with a reverse transcription-polydeoxyribonucleotide chain reaction assay to facilitate expression cloning of processed transcripts. Subsequent expression of intronless cDNAs in a suitable prokaryotic host...

With the recent advances in genomics and sequencing technologies, databases of transcriptomes representing many cellular processes have been assembled. Meiotic transcriptomes in plants have been studied in Arabidopsis thaliana, rice (Oryza sativa), wheat (Triticum aestivum), petunia (Petunia hybrida), sunflower (Helianthus annuus), and maize (Zea mays). Studies in all organisms, but particularl...

Arbuscular mycorrhiza (AM) is a mutual symbiosis that involves a complex symbiotic interface over which nutrients are exchanged between the plant host and the AM fungus. Dozens of genes in the host are required for the establishment and functioning of the interaction, among them nutrient transporters that mediate the uptake of mineral nutrients delivered by the fungal arbuscules. We have isolat...

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Vacuoles were isolated from the upper epiderm is o f petals o f Petunia hybrida line R27 using three different procedures. Utilizing the vacuolar localization o f anthocyanin, vacuolar prep­ arations obtained through polybase treatment, osm otic shock and m echanical forces were com ­ pared on purity, yield and stability. The comparison indicated that application o f the polybase procedure resu...

In the last two decades the genetic and molecular research on floral development has advanced tremendously. Initially the research focused mostly on the two species of which the homeotic floral development mutants formed the basis for the ABC‐model: Arabidopsis and Antirrhinum. In recent years the importance of studying a wider range of species, especially in an ‘‘evo‐devo’’ context, has become...

The petunia rbcS gene SSU301 was introduced into tobacco using Agrobacterium tumefaciens-mediated transformation. The time at which rbcS expression was maximal after transfer of the tobacco plants to the greenhouse was determined. The expression level of the SSU301 gene varied up to 9 fold between individual tobacco plants which had been standardized physiologically as much as possible. The pre...