32 Recently the DiversiLab (DL) system (bioMérieux) was developed as an automated platform that uses 33 rep-PCR technology for standardized, reproducible DNA fingerprinting of bacteria. The purpose of this 34 study was to evaluate the usefulness of DL rep-PCR for typing of Pseudomonas aeruginosa isolates. 35 The performance of DL rep-PCR was compared with that of pulsed-field gel electrophoresi...

AmpC is a class C Ambler β-lactamase that confers resistance to cephamycins. Globally reported AmpC-producing Enterobacterales are clinically important due their therapeutic restrictions and epidemiology. Between April 2021 May 2021, an outbreak of beta-lactamase-producing Escherichia coli occurred at Showa University Hospital. Because this outbreak, plasmid typing, enterobacterial repetitive i...

background mycobacterium tuberculosis genotyping can effectively improve tuberculosis (tb) control programs by controlling disease transmission. pulsed field gel electrophoresis (pfge) is a particularly powerful tool for determination of clonal identity of bacteria providing information for understanding and controlling the spread of disease. objectives the aim of present study was to investiga...

Campylobacter jejuni is an important foodborne pathogen with global distribution. We describe a genotyping study of collection C. (n = 137) isolated from different broiler farms and multiple sites along the processing line in slaughterhouse Argentina during 2011, 2012 2015. The isolates were genotyped using pulsed-field gel electrophoresis (PFGE) multilocus sequence typing (MLST). Based on PFGE...

This study evaluates the multiple-locus variable-number tandem-repeat assay (MLVA) and pulsed-field gel electrophoresis (PFGE) when using restriction enzymes BstZI, SacII, and ApaI to fingerprint a diverse collection of methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) sequence type 398 (ST398) isolates. These isolates had been characterized previously by multilocus sequence typin...

Many bacterial typing methods are specific for one species only, time-consuming, or poorly reproducible. DiversiLab (DL; bioMérieux) potentially overcomes these limitations. In this study, we evaluated the DL system for the identification of hospital outbreaks of a number bacterial species. Appropriately typed clinical isolates were tested with DL. DL typing agreed with pulsed-field gel electro...

We identified seven distinct subtypes of enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates that were derived from sporadic cases and outbreaks from multiple prefectures in Japan in 2005. A surveillance system utilizing pulsed-field gel electrophoresis (PFGE), PulseNet Japan, was used. Some strains showed indistinguishable PFGE patterns using another restriction enzyme (BlnI or SpeI) in...

The clonal relationship among Salmonella enterica serotype Typhimurium isolates from selected pig production units in Denmark was investigated by the pulsed field gel electrophoresis (PFGE) typing method to determine environmental survival and spread of Salmonella in different herds. Thirty-four Typhimurium isolated during 1996-1998 from porcine faeces and environmental samples from three pig f...

Identifying the sources of fecal contaminants in surface water bodies such as rivers and lakes is of significant importance for environmental quality, food safety and regulatory purposes. Current DNA library-based source tracking approaches rely on the comparison of the genetic relatedness among the fecal contaminants. The objective of this study was to determine the genetic relatedness of Esch...