Methicillin-resistant Staphylococcus aureus (MRSA) is a major human pathogen worldwide. However, data on MRSA prevalence in the African continent are scarce and nonexistent for São Tomé and Príncipe. In November 2010 and April 2012, a total of 332 individuals (258 patients and 74 health care workers [HCW]) from Hospital Dr. Ayres Menezes in São Tomé and Príncipe, were screened for S. aureus and...

A total of 18 strains of EHEC O157:H7 were isolated from distinct cases in Akita Prefecture, Japan from July to September 2007. The genetic relatedness of these isolates was investigated by performing a multilocus variable number of tandem repeats analysis (MLVA) and a pulsed-field gel electrophoresis (PFGE) analysis using XbaI. The PFGE analyses allowed us to group these 18 isolates into three...

results among the specimens, 11 cases (1.4%) were culture-positive. among these isolates, only two isolates had high mic values for erythromycin and clarithromycin. pulsed-field gel electrophoresis analysis of the isolates revealed 6 pfge profiles (a-f) among which three and two isolates had the same patterns in profiles a and b, respectively. conclusions azithromycin can be a good drug of choi...

conclusions the obtained results revealed that there has been a high rate of genetic diversity in bioluminescent strains isolated from gulf of izmir and v. lentus and v. crassostreae strains could be also bioluminescent for the first report. at the same time, pfge analysis of bioluminescent bacteria including four different genera and ten different species were shown for the first time by this ...

A total of 620 samples collected from two factories in China producing powdered infant formula (PIF) between July and November 2016 were analyzed for Cronobacter. Antimicrobial susceptibility, pulsed-field gel electropho-resis (PFGE), biofilm formation Cronobacter carried out. The results showed that 2.26% positive among the 33 isolates identified. All susceptible to 12 antimicrobial agents tes...

Rapid and sensitive methods for accurate strain delineation are essential for monitoring and preventing transmission of methicillin-resistant Staphylococcus aureus (MRSA). Pulsed-field gel electrophoresis (PFGE) has been the standard technique for strain typing most bacterial species including MRSA. The goal of this study was to compare the performance of the DiversiLab microbial typing system ...

One hundred seventy-nine Streptococcus pyogenes isolates recovered from scarlet fever patients from 1996 to 1999 in central Taiwan were characterized by emm, Vir, and pulsed-field gel electrophoresis (PFGE) typing methods. The protocols for Vir and PFGE typing were standardized. A database of the DNA fingerprints for the isolates was established. Nine emm or emm-like genes, 19 Vir patterns, and...

Escherichia coli O157:H7 is a major cause of food-borne illness in the United States. Outbreak detection involves traditional epidemiological methods and routine molecular subtyping by pulsed-field gel electrophoresis (PFGE). PFGE is labor-intensive, and the results are difficult to analyze and not easily transferable between laboratories. Multilocus variable-number tandem repeat (VNTR) analysi...

OBJECTIVES The aim of this study was to identify the profile of antibiotic resistance among E. coli O26, O103 and O145 in two cohorts of Scottish beef cattle on two farms and to determine whether there is an association between resistant phenotypes and the genotypic PFGE patterns to suggest clonality among resistant strains. METHODS MICs of 11 antibiotics for 297 E. coli O26, 152 E. coli O103...