All glycerophospholipids are made from phosphatidic acid, which, according to the traditional view, is generated at the cytosolic surface of the ER. In yeast, phosphatidic acid is synthesized de novo by two acyl-CoA-dependent acylation reactions. The first is catalysed by one of the two homologous glycerol-3-phosphate acyltransferases Gpt2p/Gat1p and Sct1p/Gat2p, the second by one of the two 1-...

Rat granulosa cells isolated from mature Graafian follicles were incubated with luteinizing hormone under various conditions in order to follow the synthesis and degradation of phospholipids. During acute incubations, luteinizing hormone provoked rapid and concentration-dependent increases in the incorporation of 32PO4 into phosphatidic acid, phosphatidylinositol, and the polyphosphoinositides....

Endocytic transport necessitates the generation of membrane tubules and their subsequent fission to transport vesicles for sorting of cargo molecules. The endocytic recycling compartment, an array of tubular and vesicular membranes decorated by the Eps15 homology domain protein, EHD1, is responsible for receptor and lipid recycling to the plasma membrane. It has been proposed that EHD dimers bi...

This paper explores the way ceramide, sphingosine, ceramide 1-phosphate, and sphingosine 1-phosphate modulate the generation of second lipid messengers from phosphatidic acid in two experimental models of the central nervous system: in vertebrate rod outer segments prepared from dark-adapted retinas as well as in rod outer segments prepared from light-adapted retinas and in rat cerebral cortex ...

Presently available evidence indicates that phosphatidic acid is an essential intermediate in the biosynthesis of glycerophosphatides in Escherichia coli. Two alternative pathways for the synthesis of phosphatidic acid in E. coli have been proposed, one involving the acylation of L-glycerol j-phosphate, and the second the direct phosphorylation of o, /3-diglyceride, catalyzed by the enzyme digl...

BACKGROUND A rare variant of Guillain-Barré syndrome (GBS) consists of facial diplegia and paresthesia, but an even more rare association is with facial hemiplegia, similar to Bell's palsy. This case report is of this rare variant of GBS that was associated with IgG antibodies to galactocerebroside and phosphatidic acid. CASE REPORT A 54-year-old man presented with lower left facial palsy and p...

The regulation of ion channels by phosphatidic acid (a proposed active metabolite in the phosphatidylinositol e f fec t ) was investigated using IH-NMR spectroscopy and smaJl unilamellar phospholipid vesicles. Transport across egg-yolk phosphatidylcholine (egg PC) and d i p a l m i t o y l phosphatidylcholine (DPPC) vesicular membranes in the presence of the channel-forming ionophores alamethic...