Raw starch degrading enzyme specially glucoamylase with binding domain (SBD) has great values in the processing industry because it digests particles below gelatinization temperature by releasing glucose from non-reducing ends sequentially. The purpose of study was to measure secretion levels recombinant Pichia pastoris, using α-mating factor signal peptide (α-MF) and native Aspergillus flavus ...

Experimental research on beetle responses to removal of logging residues following clearcut harvesting in the boreal balsam fir forest of Quebec revealed several abundant rove beetle (Staphylinidae) species potentially important for long-term monitoring. To understand the trophic affiliations of these species in forest ecosystems, it was necessary to analyze their gut contents. We used microsco...

Oral microbiota contribute to health and disease, and their disruption may influence the course of oral diseases. Here, we used pyrosequencing to characterize the oral bacteriome and mycobiome of 12 HIV-infected patients and matched 12 uninfected controls. The number of bacterial and fungal genera in individuals ranged between 8-14 and 1-9, among uninfected and HIV-infected participants, respec...

Secretory human prostatic acid phosphatase (hPAP) is glycosylated at three asparagine residues (N62, N188, N301) and has potent antinociceptive effects when administered to mice. Currently, it is unknown if these N-linked residues are required for hPAP protein stability and activity in vitro or in animal models of chronic pain. Here, we expressed wild-type hPAP and a series of Asn to Gln point ...

The yeast Cyberlindnera fabianii is used in wastewater treatment, fermentation of alcoholic beverages, and has caused blood infections. To assist in the accurate identification of this species, and to determine the genetic basis for properties involved in fermentation and water treatment, we sequenced and annotated the genome of C. fabianii (YJS4271).

The effect of red (660 nm) and far-red (730 nm) light on the stability of the yeast Candida guilliermondii to lethal u.v. radiation has been studied. Reactivation and protection were exhibited for 30 min after treatment with red light and were abolished by far-red exposure applied within this time period. The temperature dependence of the reactivation effect was also studied. The data obtained ...

MOSl uredospores of rUSl fungi develop infeclion SlrUClUres in a lypical pallern so lhal lhey can infecl lhe hosl plam. The funclion of lhese infeclion SlrUClures is divided intO lhe following three