tgiflx, a homoproteins cluster member located on the x chromosome, has a critical role in male reproduction and prostate development. previous studies have shown the erratic expression of tgiflx gene in a large proportion of prostate tumors. however tgiflx function in prostate development remains unknown. the purpose of this study was to evaluate the consequences of tgiflx expression on prostat...

conclusions the results of the present study will increase our knowledge about molecular cloning and expression of the l. infantum kmp-11 gene, and this may be used as an effective target for controlling visceral leishmaniasis. results the kmp-11 gene was successfully subcloned in pcdna3 as a eukaryotic expression vector. recombinant kmp-11 protein was confirmed by the reverse transcriptase pol...

Abstract The persistence of plasmids in bacterial populations represents a puzzling evolutionary problem with serious clinical implications due to their role the ongoing antibiotic resistance crisis. Recently, major advancements have been made toward resolving this “plasmid paradox” but mainly nonclinical context. Here, we propose an additional explanation for maintenance multidrug-resistance E...

Klebsiella species occupy a wide range of environmental and animal niches, occasionally cause opportunistic infections that are resistant to multiple antibiotics. In particular, pneumoniae ( Kpne ) has gained notoriety as major nosocomial pathogen, due principally the rise in non-susceptibility carbapenems other beta-lactam Whilst it been proposed urban water cycle facilitates transmission path...

This study investigated the effects of the size of chromosomal DNA fragments on transformation efficacy of a plasmid. In our experiment, each of three chromosomal fragment sizes were co-transformed with varying amounts of plasmid DNA and results show that the conditions, plasmid alone, plasmid with 10kb, and plasmid with 20kb fragments gave no significant differences. On the other hand, plasmid...

Efficient initiation from ammonium trifluoroacetate salts (TFA) dendritic end-groups yields well-defined poly( l -lysine) star polypeptides with 8, 16 and 32 arms. Hydrolytic core degradation plasmid DNA complexation is demonstrated.

conclusions the results of this study demonstrated that the p450 cyp141 gene was successfully cloned into a pet26b plasmid vector as an expression vector. in this paper, for the first time in iran, this gene was cloned for more purposes, including the expression and purification of the recombinant cytochrome p450 cyp141 protein. results the cloning of p450 cyp141 gene was confirmed by the enzym...

conclusions resistance to oxacillin and other antibiotics was high, and most of the isolates were found to be multi-drug resistance (mdr). plasmid analysis of representative s. aureus isolates also demonstrates the presence of a wide range of plasmid sizes, with no consistent relationship between plasmid profiles and resistance phenotypes. regular surveillance of hospital infections and monitor...