Antibiotic resistant and biofilm forming uropathogenic Enterobacteriaceae are rising. This study was conducted to evaluate the efflux pump plasmid mediated genes in ciprofloxacin (CIP) hospital acquired Escherichia coli Klebsiella pneumoniae. Also, assess anti-biofilm action of carbonyl cyanide m-chlorophenylhydrazone (CCCP). Uropathogenic E. K. pneumoniae isolates were collected from Mansoura ...

Bacterial conjugative plasmids could carry genes involved in pesticide biodegradation. However, its conjugal transfer is affected by stressors such as metals. Our aim was to determine if bacterial cells exposed copper can conjugation the catabolic plasmid pRO101 and assessed expression of traK gene. Cupriavidus pinatubonensis AEO106 Pseudomonas putida KT2440 were used donor recipient strains, r...

This study was aimed to determine the prevalence of ESBLs in E. coli and their relation plasmid profiling patterns a sample patients Iraqi hospitals. The total number isolates 91 (49%) were collected out 185 clinical samples. antibiotic sensitivity test for showed high resistance percentage Nitrofurantion 95.7%, amoxicillin Ceftriaxone each which 91.3% Ceftazidime 90.2%, while highest observed ...

The stable inheritance of bacterial plasmids is achieved by a number of different mechanisms. Among them are resolution of plasmid oligomers into monomers, active plasmid partitioning into dividing cells and selective killing of plasmid-free segregants. A special focus is given to the last mechanism. It involves a stable toxin and an unstable antidote. The antidotes neutralize their cognate tox...

plasmid DNA. Those fragments represent the homologous Ti-plasmid DNA segment 8,um long (1 5 % of the Ti-plasmid). An argument that this region of the plasmid contains genes essential for the expression of oncogenicity came from the analysis of a deletion mutant in the Ti-plasmid (Hernalsteens et al., 1975). Since this deletion mutant is unable to confer oncogenicity and has lost exactly those t...

Background & Aims: Emergence of biofilm producing Proteus strains created a serious problem in the treatment of catheter-associated urinary tract infections. The aim of this research was to study biofilm production and plasmid pattern of proteus strains associated with Urinary tract infection. Methods: A total of 88 strains of Proteus were isolated from samples collected in hospitals of Kerman/...

Although plasmid DNA vectors have been extensively applied in biotechnology, there is still a lack of standard plasmid vector classification. Here, we propose a classification method for commonly used plasmid vectors. Plasmid vectors were classified into different classes based on their replication origin, selection marker and promoter information. The replication origins of plasmid vectors wer...

Low copy number plasmids in bacteria require segregation for stable inheritance through cell division. This is often achieved by a parABC locus, comprising an ATPase ParA, DNA-binding protein ParB and a parC region, encoding ParB-binding sites. These minimal components space plasmids equally over the nucleoid, yet the underlying mechanism is not understood. Here we investigate a model where Par...

Large conjugative plasmids are important drivers of bacterial evolution and contribute significantly to the dissemination of antibiotic resistance. Although plasmid borne multidrug resistance is recognized as one of the main challenges in modern medicine, the adaptive forces shaping the evolution of these plasmids within pathogenic hosts are poorly understood. Here we study plasmid-host adaptat...