toxoplasmosis is a worldwide infection which is commonly asymptomatic but can cause serious medical problems in immunocompromised individuals and fetus. the infection also causes considerable economic loss because of abortion in livestock, mostly in sheep and goats. dna vaccination may be a powerful approach against intracellular parasites such as toxoplasma gondii. the goal of this study was t...

Abstract In April 2019, hydroponically cultivated cucumber plants with characteristic symptoms of crazy root disease were found in two different commercial production greenhouses Poland. Due to excessive and inappropriate growth, this led a reduction yield deterioration the general conditions infected plants. Bacteria isolated from roots subjected morphological evaluation, as well molecular, bi...

Plasmid toxins CcdB and ParE are part of addiction systems promoting plasmid maintenance. Both target host DNA gyrase, as do quinolones and plasmid-determined Qnr proteins that protect gyrase from quinolone inhibition. We cloned qnrB4, qnrS1, ccdB, parE, and the antitoxin-encoding genes ccdA and parD on compatible plasmids and tested them in combination. CcdB and ParE had no specific effect on ...

introduction: resistance of pathogenic organisms to countenance antibiotics has become a worldwide problem with serious consequences on the treatment of infectious diseases. the aim of this study was to evaluate antibiotic resistance and also the detection of transferred antibiotic resistance by plasmid in clinical proteus isolates. materials and methods: a total of 250 urine samples were colle...

background: alpinia galanga l. belonging to the family zingiberaceae is widely grown in the state of kerala, india. they are effective antioxidant and free radical scavenger under both in vitro and in vivo condition. the efficacy of the isolated flavonoids in conferring protection from radiation induced damages to genomic dna was studied. materials and methods: the bioflavonoids, galangin and k...

gene transfer to plants and the production of transgenic plants with various purposes, such as improving the performance and quality, resistance to pests, diseases, etc., and of great importance are carried out gene transfer to plants performs to evaluate the transient and permanent gene expression. transient expression is quick, easy and simple and is not influenced by position effect compare ...

Background: Urinary tract Infections caused by multidrug resistant uropathogens have become a significant global public health problem with Nigeria being no exception.
 Objective: This study is aimed at profiling and curing the plasmids of selected isolated from patients urinary infection in tertiary hospital Calabar, Nigeria.
 Methodology: Isolates were obtained urine samples using s...

Transcription initiation of the copy-number control and better-than-random segregation genes of the broad-host-range and low-copy-number plasmid pSM19035 are subjected to repression by the autoregulated pSM19035-encoded omega product in Bacillus subtilis cells. The promoters of the copS (Pcop1 and Pcop2), delta (Pdelta), and omega (Pomega) genes have been mapped. These promoters are embedded in...

Background: Escherichia coli is still the common host for ing and heterologous protein expression. Various strategies have been employed to increase protein expression in E. coli, but, it seems that external factors such as selection marker concentration can drastically aff ect the yield of protein and plasmid.Objectives: Alterations of protein expression...

Abstract Inappropriate use of antibiotics is globally creating public health hazards associated with antibiotic resistance. Bacteria often acquire resistance by altering their genes through mutation or acquisition plasmid-encoding genes. To treat drug-resistant strains bacteria, the recently developed CRISPR-Cas9 system might be an alternative molecular tool to conventional antibiotics. It disa...