objective: evaluation of bax encoding plasmid for increasing efficacy of dna vaccine plasmid encoding gb of herpes simplex virus type 1. materials and methods: we compared three different dosages of bax encoding plasmid (pcbax) including 10, 25 and 50 μg of plasmid dna. they were co-injected ineradermally with glycoprotein b (gb) of herpes simplex virus (hsv)-1 encoded plasmid (pcgb) in c57bl/6...

introduction: neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. also, neurotropic factors are growth factors like neurotrophins that help neurons survive. moreover, neurotrophins differentiate between progenitor cells so that neurons are formed. despite the fact that the majority of mammalian brain neurons are produced p...

Abstract The emergence and dissemination of colistin resistance in Enterobacteriaceae mediated by plasmid-borne mcr genes recent years now pose a threat to public health. In this study, we isolated characterized colistin-resistant and/or -positive E. coli from pig farms Central China. Between 2018 2019, 594 samples were collected recovered 445 isolates. Among them, 33 with phenotypes 37 that po...

The mcr-1 gene mediating mobile colistin resistance in Escherichia coli was first reported China 2016 followed by reports among different species worldwide, especially E. and Klebsiella . However, data on its transmission Salmonella are still lacking. This study analyzed the antimicrobial (AMR) profiles presence 755 foodborne from 26 provinces of mainland, 2016. Genomic features two mcr-1- carr...

No recombination between plasmids was observed after conjugal transfer of a plasmid into a cell carrying another plasmid. Two types of such recombination took place after transformation, one type being Rec+ dependent and suggesting a preferred site of recombination. The other much rarer type was at least partially Rec+ independent.

human interferon β (inf-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. in this study an expression vector was designed and constructed for expression of human inf-β-1b either in shake flasks and bench top bioreactor. the designed vector was constructed based upon pet-25b(+) with t7 promoter. recombinant human beta interferon ...