the biosynthetic pathways of saturated and unsaturated fatty acids consist of many steps controlled by various enzymes. one of the methods for improving oil quality is to change the fatty acid profile through genetic manipulation which requires isolation and characterization of the genes and other cis-acting elements, such as the promoter, involved in fatty acid biosynthesis. b-ketoacyl-coa syn...

implantation failure is the most frequent cause of pregnancy loss in couples who try to conceive, either in a natural way or using assisted reproductive techniques (art). identify the precise mechanisms of implantation failure can lead to identify couples at risk and also providing appropriate therapeutic options to affected couples. despite the high prevalence of this disorder, a few causing f...

background & target: ischemia reperfusion injury is the tissue damage caused when blood supply returns to the tissue after a period of ischemia or lack of oxygen. in this study, the effect of pentoxyfylline on bcl2 gene expression changes and cell injury in kidney of rat following ischemia reperfusion were evaluated.methods: in this experimental study, 20 male wistar rats with average weight of...

introduction: β-globin gene cluster haplotypes are useful in diagnosis of particular molecular defects in β-thalassemia, prenatal diagnosis of β-thalassemia, and elucidating population affinities. methods: β-globin gene cluster haplotypes were studied in 150 β-thalassemia minor and 52 healthy in-dividuals from the fars province of iran. dna was extracted from leukocytes of whole blood by phe-no...

cryptosporidium is an apicomplexan parasite of humans and a wild range of domestic as well as wild animals. an 833-bp fragment of the 18s-rrna gene was used to identify cryptosporidium spp. recovered from children and adult patients, in human immunodeficiency virus (hiv) positive and negative patients in iran. initial identification of cryptosporidiosis was carried out by ziehl-neelsen acid-fas...

background:it has been hypothesized that genetic factors other than histocompatibility disparity may play a role in predisposition to developing chronic myelogenous leukemia (cml). in this regard, th1 and th2 cytokines and their gene polymorphism seems to be important. overall expression and secretion of cytokines is dependent, at least in part, on genetic polymorphism (nucleotide variations) w...

background: nowadays, oocytes and embryos vitrification has become a routine technique. based on clinical judgment, re-vitrification maybe required. but little is known about re-vitrification impact on genes expression. objective: the impact of re-vitrification on apoptotic and implanting genes, bax, bcl-2 and erbb4, at compaction stage embryos were evaluated in this study. materials and method...

Lysyl hydroxylase 3 (LH3) has lysyl hydroxylase, galactosyltransferase, and glucosyltransferase activities, which are sequentially required for the formation of glucosylgalactosyl hydroxylysines in collagens. Here we demonstrate for the first time that LH3 also modifies the lysine residues in the collagenous domain of adiponectin, which has important roles in glucose and lipid metabolism and in...

Lysyl hydroxylase (EC1.14.11.4, LH) catalyzes post-translationally the hydroxylation of lysyl residues in collagens and other proteins with collagenous domains. Hydroxylysyl residues may also be glycosylated by hydroxylysyl galactosyltransferase (EC 2.4.1.50, GT) or galactosylhydroxylysyl glucosyltransferase (EC 2.4.1.66, GGT) to form galactosylhydroxylysyl or glucosylgalactosylhydroxylysyl res...