Nuclear suspensions of 42 prostate carcinoma specimens obtained at surgery were used to investigate loss and gain chromosomes 1, 18, and Y by fluorescence in situ hybridization (FISH) with centromere-specific probes. The outcome of FISH analysis was correlated with clinical parameters and the relationship between DNA-FCM (ploidy at cellular level) and FISH (ploidy of individual chromosomes) was...

BACKGROUND The objective of the current pilot project was to assess the efficacy of laser scanning cytometry (LSC) for DNA ploidy analysis of atypical urologic cytology specimens to enhance the distinction between benign and malignant changes. METHODS Forty selected urologic cytology specimens that previously had been categorized as normal, atypical, or malignant were studied. Nuclear propidi...

BACKGROUND For uveal melanoma it has been demonstrated that aneuploidy correlates with worse clinical outcome. However, a striking variation in incidence of aneuploidy is reported for uveal melanomas. METHODS Flow cytometry was used to study retrospectively DNA-ploidy of 132 uveal melanomas on paraffin embedded material. Thirty five patients received 2 x 4 Gy doses of irradiation 24 and 48 ho...

This study investigates species boundaries in the polyploid complex of Rosa sect. Cinnamomeae east of the Rocky Mountains. This complex is characterized by extensive intra-specific polymorphism that is the consequence, in part, of hybridization and polyploidy. An objective multivariate approach is employed to delimit species in the complex, which involved cluster and ordination analyses of 25 q...

DNA ploidy and chromosome (FISH) pattern analysis of peripheral nerve sheath tumors To the Editor, This report contains highly informative image DNA cytometry and Fluorescence in situ Hybridization (FISH) data on the DNA ploidy analysis of peripheral nerve sheath tumors [3]. The FISH analysis used DNA probes specific for centromeric regions combined with automatic image analysis. The data suppo...

One of the most efficient mechanisms to keep animal lineages separate is a difference in ploidy level (number of whole genome copies), since hybrid offspring from parents with different ploidy level are functionally sterile. In the freshwater fish family Botiidae, ploidy difference has been held responsible for the separation of its two subfamilies, the evolutionary tetraploid Botiinae and the ...

Chromosome numbers are reported for 112 collections of Cactaceae, including 102 collections of Opuntia. Reports are presented for 33 species in five genera and a total of 36 taxa. Two specimens were sterile. Emphasis was given to the documentation of intraspecific ploidy-level consistencies and differences, because of the current poor state of knowledge about the populational integrity of chrom...

Ploidy varies tremendously within and between species, yet the factors that influence when or why ploidy variants are adaptive remains poorly understood. Our previous work found that diploid individuals repeatedly arose within ten replicate haploid populations of Saccharomyces cerevisiae, and in each case we witnessed diploid takeover within ~1800 asexual generations of batch culture evolution ...

Twenty-five radical prostatectomy specimens were screened for the presence of numerical chromosome changes within the adenocarcinoma as well as 17 adjacent prostatic intraepithelial neoplasias (PIN) by means of interphase in situ hybridization (ISH) to routinely processed tissue sections. To this end a defined alfoid repetitive DNA probe set was used, specific for the centromeres of chromosomes...

PURPOSE To explore whether DNA ploidy of prostate cancer cells determined from archived transrectal ultrasound-guided biopsy specimens correlates with disease-free survival. METHODS AND MATERIALS Forty-seven failures and 47 controls were selected from 1006 consecutive low- and intermediate-risk patients treated with prostate (125)I brachytherapy (July 1998-October 2003). Median follow-up was ...