Chloroplasts of Nicotiana tabacum SR1 were transferred into Nicotiana plumbaginifolia by protoplast fusion. The protoplasts of the organelle donor were irradiated with different lethal doses using a (60)Co source, to facilitate the elimination of their nuclei from the fusion products. After fusion induction, clones derived from fusion products and containing streptomycin-resistant N. tabacum SR...

EVERAL workers have already reported that hybridization between Nicotiana species can bring about chromosome instability. One conspicuous case is loss of somatic plumbaginifolia chromosomes in derivatives of N . tabacum X N . plumbaginifolia hybrids (MOAV 1961). The present paper deals with another peculiarity of alien chromosomes in tobacco hybrid derivatives. It reports on heritable changes i...

HE origin of chromosome instability and subsequent chlorophyll variegation Tin hybrid derivatives of Nicotiana tabacum (n=24) and N . plumbaginifolia (n=10) has been described by MOAV (1961) and GUPTA (1965, 1968b). It was demonstrated by (GUPTA 1968b) that the alien chromosome of N . plumbaginifolia (pbg) carrying a Ws gene (chlorophyll factor) showed instability in association with the N . ta...

Genetic recombination between chloroplasts of two flowering plant species, Nicotiana tabacum and Nicotiana plumbaginifolia, after somatic cell fusion is described. The parental lines differed in three cytoplasmic genetic markers. The N. tabacum mutant SR1-A15 was streptomycin-resistant, defective in chloroplast greening, and lincomycin-sensitive. The N. plumbaginifolia mutant LR400 was streptom...

The best way to control mosquito borne diseases is to control the mosquito population. The present study was executed to evaluate the mosquito larvicidal activity of Nicotiana plumbaginifolia leaves against malarial vector Anopheles stephensi.Crude extracts and three different solvent extracts were bio-assayed against An. stephensi larvae. LC50 and LC90 values were determined appropriately. Qua...

Healthy buds of Nicotiana plumbaginifolia growing in the Kashmir University Botanic Garden were selected for the present study. Flower development and senescence was divided into seven stages, viz., tight bud stage (I), mature bud stage (II), pencil stage (III), partially open stage (IV), open stage (V), partially senescent stage (VI) and senescent stage (VII). Various physiological and biochem...

The sensitivity of the Nicotiana plumbaginifolia wild-type and phytochrome B (hlg) mutant to photoperiod was investigated. Leaf number at bolting and rosette-leaf movement were measured under conditions of different light quality and photoperiod. Bolting in wild-type plants was slightly delayed under short days (SD), compared to plants grown under long days (LD). The hlg mutant bolted later tha...