Prolonged exposure to an oriented line shifts the perceived orientation of a subsequently observed line in the opposite direction, a phenomenon known as the tilt aftereffect (TAE). Here we consider whether the TAE for line stimuli is mediated by a mechanism that integrates the local parts of the line into a single global entity prior to the site of adaptation, or the result of the sum of local ...

Mitochondrial polarity is a crucial characteristic of these indispensable organelles, and tremendously impacts cellular events. Herein, we describe a new mitochondria-targeting fluorescent probe MCY-BF2 that is singularly sensitive and specifically responsive to mitochondrial polarity. The pull–push system in the conjugated structure of MCY-BF2 is responsible for the polarity-ultrasensitivity d...

We used Gaussian blurred lines of same- and opposite-polarity to measure the effects of blur on 3-line spatial interval discrimination (bisection). The results of our experiments can be summarized as follows. Spatial interval discrimination (3-line bisection) thresholds are proportional to the separation of the lines (i.e. Weber's law). At the optimal separation, spatial interval discrimination...

Alignment of the mitotic spindle to the cellular polarity axis is a prerequisite for asymmetric cell divisions. The protein network coordinating the spindle position with cortical polarity includes the molecular machinery pulling on astral microtubules, which is assembled on conserved NuMA:LGN:Gαi complexes, the polarity proteins Par3:Par6:aPKC and an adaptor molecule known as Inscuteable (Insc...

We describe methods for live-cell imaging of yeast cells that we have exploited to image yeast polarity establishment. As a rare event occurring on a fast time-scale, imaging polarization involves a trade-off between spatiotemporal resolution and long-term imaging without excessive phototoxicity. By synchronizing cells in a way that increases resistance to photodamage, we discovered unexpected ...

This paper presents work on the automatic creation of a polarity lexicon based on a lexical-semantic network. During this work, we noticed that the language registers of a relation should be considered in polarity propagation. After analysing the possible registers and performing some experiments, our intuition was confirmed – there are registers that should invert the transmitted polarity (iro...

Current state of emotive annotation of plWordNet: •more than 83k annotations covering more than 54k lexical units and 41k synsets • 22k polarity annotations different than neutral (13k of lexical units and 9k synsets) • 1.5k synsets with different polarity across their units –without neutral units, only 345 of synsets with varying polarity strength –without neutral and ambiguous annotations, on...